Quantification of diacylglycerol molecular species in biological samples by electrospray ionization mass spectrometry after one-step derivatization

Ying L. Li, Xiong Su, Philip D. Stahl, Michael L. Gross

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Diacylgtycerols (DAGs) are important lipid intermediates in cellular trafficking and signaling. Their concentrations are altered in diabetes, cancer, and other disease states. Quantification of DAGs in biological samples may provide critical information to uncover molecular mechanisms leading to various cellular functional disorders. Recent advances in lipidomics using mass spectrometry have greatly accelerated global lipid analysis and quantification. Quantification of DAGs by electrospray mass spectrometry (ESI/MS), however, is challenged by the absence of a permanent charge on the molecule, its low proton affinity and acidity, and its low abundance under normal biological conditions. We describe here the introduction of a quaternary ammonium cation to DAG molecules, using N-chlorobetainyl chloride, to afford a derivatized DAG that gives 2 orders of magnitude higher signal intensities than their underivatized sodium adducts. A linear calibration curve in which peak intensity ratios are plotted versus molar ratios can be achieved by using ESI/MS with dilauroyl glycerol as the internal standard. Employing this new approach to this analyte, we found a 9-fold increase of total DAGs in the livers of obese db/db mice as compared to their heterozygous lean controls. This proven strategy can be used to detect and quantify DAG molecular species from biological samples using ESI/MS after one-step derivatization.

Original languageEnglish
Pages (from-to)1569-1574
Number of pages6
JournalAnalytical Chemistry
Volume79
Issue number4
DOIs
StatePublished - Feb 15 2007

Fingerprint Dive into the research topics of 'Quantification of diacylglycerol molecular species in biological samples by electrospray ionization mass spectrometry after one-step derivatization'. Together they form a unique fingerprint.

  • Cite this