Quantification of choline and ethanolamine phospholipids in rabbit myocardium

Michael H. Creer; Christine Pastor; Peter B. Corr; Richard W. Gross; Burton E. Sobel

doi:10.1016/0003-2697(85)90084-3

Quantification of choline and ethanolamine phospholipids in rabbit myocardium

Michael H. Creer, Christine Pastor, Peter B. Corr, Richard W. Gross, Burton E. Sobel

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14 Scopus citations

Abstract

To facilitate evaluation of the influence of myocardial phospholipid metabolities on the development of electrophysiologic abnormalities induced by ischemia, a method for the quantification of choline and ethanolamine phospholipids suitable for accurate and reproducible analysis of small amounts of myocardium was developed. The procedure combines chloroform and methanol extraction of phospholipids after tissue homogenization with subsequent separation by sequential thin-layer and high-performance liquid chromatography. Phosphorus in purified lipid classes was determined with the correction for recovery based on ¹⁴C-labeled internal standards.

Original language	English
Pages (from-to)	65-74
Number of pages	10
Journal	Analytical Biochemistry
Volume	144
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(85)90084-3
State	Published - Jan 1985

Keywords

HPLC
TLC
lipids
myocardium
phosphate assay
phospholipids
solvent extraction

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10.1016/0003-2697(85)90084-3

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title = "Quantification of choline and ethanolamine phospholipids in rabbit myocardium",

abstract = "To facilitate evaluation of the influence of myocardial phospholipid metabolities on the development of electrophysiologic abnormalities induced by ischemia, a method for the quantification of choline and ethanolamine phospholipids suitable for accurate and reproducible analysis of small amounts of myocardium was developed. The procedure combines chloroform and methanol extraction of phospholipids after tissue homogenization with subsequent separation by sequential thin-layer and high-performance liquid chromatography. Phosphorus in purified lipid classes was determined with the correction for recovery based on 14C-labeled internal standards.",

keywords = "HPLC, TLC, lipids, myocardium, phosphate assay, phospholipids, solvent extraction",

author = "Creer, {Michael H.} and Christine Pastor and Corr, {Peter B.} and Gross, {Richard W.} and Sobel, {Burton E.}",

note = "Funding Information: {\textquoteright} Supported in part by NIH Grant HL17646, SCOR in Ischemic Heart Disease.",

year = "1985",

month = jan,

doi = "10.1016/0003-2697(85)90084-3",

language = "English",

volume = "144",

pages = "65--74",

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T1 - Quantification of choline and ethanolamine phospholipids in rabbit myocardium

AU - Creer, Michael H.

AU - Pastor, Christine

AU - Corr, Peter B.

AU - Gross, Richard W.

AU - Sobel, Burton E.

N1 - Funding Information: ’ Supported in part by NIH Grant HL17646, SCOR in Ischemic Heart Disease.

PY - 1985/1

Y1 - 1985/1

N2 - To facilitate evaluation of the influence of myocardial phospholipid metabolities on the development of electrophysiologic abnormalities induced by ischemia, a method for the quantification of choline and ethanolamine phospholipids suitable for accurate and reproducible analysis of small amounts of myocardium was developed. The procedure combines chloroform and methanol extraction of phospholipids after tissue homogenization with subsequent separation by sequential thin-layer and high-performance liquid chromatography. Phosphorus in purified lipid classes was determined with the correction for recovery based on 14C-labeled internal standards.

AB - To facilitate evaluation of the influence of myocardial phospholipid metabolities on the development of electrophysiologic abnormalities induced by ischemia, a method for the quantification of choline and ethanolamine phospholipids suitable for accurate and reproducible analysis of small amounts of myocardium was developed. The procedure combines chloroform and methanol extraction of phospholipids after tissue homogenization with subsequent separation by sequential thin-layer and high-performance liquid chromatography. Phosphorus in purified lipid classes was determined with the correction for recovery based on 14C-labeled internal standards.

KW - HPLC

KW - TLC

KW - lipids

KW - myocardium

KW - phosphate assay

KW - phospholipids

KW - solvent extraction

UR - http://www.scopus.com/inward/record.url?scp=0021913749&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(85)90084-3

DO - 10.1016/0003-2697(85)90084-3

M3 - Article

C2 - 3985327

AN - SCOPUS:0021913749

SN - 0003-2697

VL - 144

SP - 65

EP - 74

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

Quantification of choline and ethanolamine phospholipids in rabbit myocardium

Abstract

Keywords

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