Quantification of β-galactosidase activity after non-viral transfection in vivo

Bernd H. Zinselmeyer, Nicola Beggbie, Ijeoma F. Uchegbu, Andreas G. Schätzlein

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The limited efficacy of non-viral gene delivery systems currently hampers their wider therapeutic use. In order to further develop novel gene delivery systems, it is important to quantify their efficacy. Many reporter gene assays have limitations when being used to quantify expression in vivo. We have developed a simple assay which allows the quantification of β- galactosidase transgene activity in vivo. The assay is based on β-galactosidase cleavage of the DDAO-galactopyranoside substrate to DDAO, which shifts the fluorescence towards longer wavelengths. Reaction conditions were optimised to minimise degradation, activity of endogenous β-galactosidase, and non-specific background fluorescence. The spectrofluorimetric quantification of the reaction product DDAO in the red part of the spectrum avoided interference from haemoglobin or other bio-molecules which hamper many in vivo assays. Routinely, amounts of less than 1 ng of β-galactosidase (1 mU) per gram tissue could be detected and quantified. After intravenous administration of β-galactosidase complexed with linear polyethylenimine (PEI, 22 kD) in mice, 134 mU g-1 β-galactosidase were detected in the lung, but only 2.9 mU g-1 were found in the liver.

Original languageEnglish
Pages (from-to)201-208
Number of pages8
JournalJournal of Controlled Release
Volume91
Issue number1-2
DOIs
StatePublished - Aug 28 2003

Keywords

  • Fluorescence assay
  • Gene expression
  • In vivo
  • Reporter gene
  • β-Galactosidase

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