Purification of human brain tissue factor

G. J. Broze; J. E. Leykam; B. D. Schwartz; J. P. Miletich

Purification of human brain tissue factor

G. J. Broze, J. E. Leykam, B. D. Schwartz, J. P. Miletich

Division of Hematology

Research output: Contribution to journal › Article › peer-review

116 Scopus citations

Abstract

Tissue factor (factor III) is a lipoprotein cofactor which markedly enhances the catalytic effect of coagulation factor VII(a) upon factors IX and X. Human tissue factor apoprotein was purified 53,000-fold to homogeneity from brain using acetone delipidation, Triton X-100 extraction, and affinity chromatography upon factor VII-agarose. The purified apoprotein has an apparent molecular weight of 44,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, an amino acid composition similar to bovine brain tissue factor, and an NH₂-terminal amino acid sequence of Ser-X-Asn-Thr-Val-Ala-Val-Tyr-X-Tyr-X-Leu-Lys-(Ser)-Lys-Asn-Phe. Optimal relipidation of the tissue factor apoprotein was associated with a 5000-fold enhancement of clotting activity and occurred at a phospholipid/apoprotein (w/w) ratio of > 600.

Original language	English
Pages (from-to)	10917-10920
Number of pages	4
Journal	Journal of Biological Chemistry
Volume	260
Issue number	20
State	Published - 1985

Cite this

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title = "Purification of human brain tissue factor",

abstract = "Tissue factor (factor III) is a lipoprotein cofactor which markedly enhances the catalytic effect of coagulation factor VII(a) upon factors IX and X. Human tissue factor apoprotein was purified 53,000-fold to homogeneity from brain using acetone delipidation, Triton X-100 extraction, and affinity chromatography upon factor VII-agarose. The purified apoprotein has an apparent molecular weight of 44,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, an amino acid composition similar to bovine brain tissue factor, and an NH2-terminal amino acid sequence of Ser-X-Asn-Thr-Val-Ala-Val-Tyr-X-Tyr-X-Leu-Lys-(Ser)-Lys-Asn-Phe. Optimal relipidation of the tissue factor apoprotein was associated with a 5000-fold enhancement of clotting activity and occurred at a phospholipid/apoprotein (w/w) ratio of > 600.",

author = "Broze, {G. J.} and Leykam, {J. E.} and Schwartz, {B. D.} and Miletich, {J. P.}",

year = "1985",

language = "English",

volume = "260",

pages = "10917--10920",

journal = "Journal of Biological Chemistry",

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TY - JOUR

T1 - Purification of human brain tissue factor

AU - Broze, G. J.

AU - Leykam, J. E.

AU - Schwartz, B. D.

AU - Miletich, J. P.

PY - 1985

Y1 - 1985

N2 - Tissue factor (factor III) is a lipoprotein cofactor which markedly enhances the catalytic effect of coagulation factor VII(a) upon factors IX and X. Human tissue factor apoprotein was purified 53,000-fold to homogeneity from brain using acetone delipidation, Triton X-100 extraction, and affinity chromatography upon factor VII-agarose. The purified apoprotein has an apparent molecular weight of 44,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, an amino acid composition similar to bovine brain tissue factor, and an NH2-terminal amino acid sequence of Ser-X-Asn-Thr-Val-Ala-Val-Tyr-X-Tyr-X-Leu-Lys-(Ser)-Lys-Asn-Phe. Optimal relipidation of the tissue factor apoprotein was associated with a 5000-fold enhancement of clotting activity and occurred at a phospholipid/apoprotein (w/w) ratio of > 600.

AB - Tissue factor (factor III) is a lipoprotein cofactor which markedly enhances the catalytic effect of coagulation factor VII(a) upon factors IX and X. Human tissue factor apoprotein was purified 53,000-fold to homogeneity from brain using acetone delipidation, Triton X-100 extraction, and affinity chromatography upon factor VII-agarose. The purified apoprotein has an apparent molecular weight of 44,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, an amino acid composition similar to bovine brain tissue factor, and an NH2-terminal amino acid sequence of Ser-X-Asn-Thr-Val-Ala-Val-Tyr-X-Tyr-X-Leu-Lys-(Ser)-Lys-Asn-Phe. Optimal relipidation of the tissue factor apoprotein was associated with a 5000-fold enhancement of clotting activity and occurred at a phospholipid/apoprotein (w/w) ratio of > 600.

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M3 - Article

C2 - 3928626

AN - SCOPUS:0022212864

SN - 0021-9258

VL - 260

SP - 10917

EP - 10920

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 20

ER -

Purification of human brain tissue factor

Abstract

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