Purification and partial characterization of (6-4) photoproduct DNA photolyase from Xenopus laevis

Sang Tae Kim; Khushbeer Malhotra; John Stephen Taylor; Aziz Sancar

doi:10.1111/j.1751-1097.1996.tb03028.x

Purification and partial characterization of (6-4) photoproduct DNA photolyase from Xenopus laevis

Sang Tae Kim, Khushbeer Malhotra, John Stephen Taylor, Aziz Sancar

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

The (6-4) photoproduct DNA photolyase was detected in two vertebrate animals Crotalus atrox (rattlesnake) and Xenopus laevis (South African clawed toad). The enzyme was extensively purified from X. laevis and characterized. The highly purified enzyme is fluorescent with an excitation maximum at 420-440 nm and emission maximum at 460-480 nm. The photorepair action spectrum matches the fluoresoence excitation spectrum with a 430 nm maximum.

Original language	English
Pages (from-to)	292-295
Number of pages	4
Journal	Photochemistry and Photobiology
Volume	63
Issue number	3
DOIs	https://doi.org/10.1111/j.1751-1097.1996.tb03028.x
State	Published - Mar 1996

Access to Document

10.1111/j.1751-1097.1996.tb03028.x

Cite this

@article{1ec537995fb0441abf4b879a2693328a,

title = "Purification and partial characterization of (6-4) photoproduct DNA photolyase from Xenopus laevis",

abstract = "The (6-4) photoproduct DNA photolyase was detected in two vertebrate animals Crotalus atrox (rattlesnake) and Xenopus laevis (South African clawed toad). The enzyme was extensively purified from X. laevis and characterized. The highly purified enzyme is fluorescent with an excitation maximum at 420-440 nm and emission maximum at 460-480 nm. The photorepair action spectrum matches the fluoresoence excitation spectrum with a 430 nm maximum.",

author = "Kim, {Sang Tae} and Khushbeer Malhotra and Taylor, {John Stephen} and Aziz Sancar",

year = "1996",

month = mar,

doi = "10.1111/j.1751-1097.1996.tb03028.x",

language = "English",

volume = "63",

pages = "292--295",

journal = "Photochemistry and Photobiology",

issn = "0031-8655",

number = "3",

}

TY - JOUR

T1 - Purification and partial characterization of (6-4) photoproduct DNA photolyase from Xenopus laevis

AU - Kim, Sang Tae

AU - Malhotra, Khushbeer

AU - Taylor, John Stephen

AU - Sancar, Aziz

PY - 1996/3

Y1 - 1996/3

N2 - The (6-4) photoproduct DNA photolyase was detected in two vertebrate animals Crotalus atrox (rattlesnake) and Xenopus laevis (South African clawed toad). The enzyme was extensively purified from X. laevis and characterized. The highly purified enzyme is fluorescent with an excitation maximum at 420-440 nm and emission maximum at 460-480 nm. The photorepair action spectrum matches the fluoresoence excitation spectrum with a 430 nm maximum.

AB - The (6-4) photoproduct DNA photolyase was detected in two vertebrate animals Crotalus atrox (rattlesnake) and Xenopus laevis (South African clawed toad). The enzyme was extensively purified from X. laevis and characterized. The highly purified enzyme is fluorescent with an excitation maximum at 420-440 nm and emission maximum at 460-480 nm. The photorepair action spectrum matches the fluoresoence excitation spectrum with a 430 nm maximum.

UR - http://www.scopus.com/inward/record.url?scp=0030111351&partnerID=8YFLogxK

U2 - 10.1111/j.1751-1097.1996.tb03028.x

DO - 10.1111/j.1751-1097.1996.tb03028.x

M3 - Article

C2 - 8881333

AN - SCOPUS:0030111351

SN - 0031-8655

VL - 63

SP - 292

EP - 295

JO - Photochemistry and Photobiology

JF - Photochemistry and Photobiology

IS - 3

ER -

Purification and partial characterization of (6-4) photoproduct DNA photolyase from Xenopus laevis

Abstract

Access to Document

Other files and links

Fingerprint

Cite this