Purification and partial characterization of (6-4) photoproduct DNA photolyase from Xenopus laevis

Sang Tae Kim; Khushbeer Malhotra; John Stephen Taylor; Aziz Sancar

doi:10.1111/j.1751-1097.1996.tb03028.x

Purification and partial characterization of (6-4) photoproduct DNA photolyase from Xenopus laevis

Sang Tae Kim, Khushbeer Malhotra, John Stephen Taylor, Aziz Sancar

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Abstract

The (6-4) photoproduct DNA photolyase was detected in two vertebrate animals Crotalus atrox (rattlesnake) and Xenopus laevis (South African clawed toad). The enzyme was extensively purified from X. laevis and characterized. The highly purified enzyme is fluorescent with an excitation maximum at 420-440 nm and emission maximum at 460-480 nm. The photorepair action spectrum matches the fluoresoence excitation spectrum with a 430 nm maximum.

Original language	English
Pages (from-to)	292-295
Number of pages	4
Journal	Photochemistry and Photobiology
Volume	63
Issue number	3
DOIs	https://doi.org/10.1111/j.1751-1097.1996.tb03028.x
State	Published - Mar 1996

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10.1111/j.1751-1097.1996.tb03028.x

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abstract = "The (6-4) photoproduct DNA photolyase was detected in two vertebrate animals Crotalus atrox (rattlesnake) and Xenopus laevis (South African clawed toad). The enzyme was extensively purified from X. laevis and characterized. The highly purified enzyme is fluorescent with an excitation maximum at 420-440 nm and emission maximum at 460-480 nm. The photorepair action spectrum matches the fluoresoence excitation spectrum with a 430 nm maximum.",

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Purification and partial characterization of (6-4) photoproduct DNA photolyase from Xenopus laevis

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