TY - JOUR
T1 - Punctuated cell cycling in the regeneration blastema of urodele amphibians
T2 - An hypothesis
AU - Tomlinson, Bruce L.
AU - Goldhamer, David J.
AU - Barger, Philip M.
AU - Tassava, Roy A.
PY - 1985/1/1
Y1 - 1985/1/1
N2 - We previously assumed that all cells in the regeneration blastema are randomly distributed throughout the cell cycle and actively cycling towards the next mitotic division. We now show that data from continuous labeling (3H-thymidine) experiments do not support this view and favor instead the hypothesis that the blastema cell cycle is punctuated in the g1 phase wherein cells can enter what we term a transiently quiescent (TQ) position. We call this hypothesis the punctuated cycling (PC) hypothesis. We further propose that the relative sizes of the quiescent and actively cycling populations explain (1) variations in rates of regeneration in different sizes of urodele amphibians, (2) the rate and success of regeneration in different species, and (3) how various controlling factors, such as injury, nerves, growth factors, wound epidermis, and hormones, influence the initiation and progression of the regeneration process. This PC hypothesis is important for interpreting previous pulse labeling data, is consistent with recently obtained continuous labeling data, and is experimentally testable.
AB - We previously assumed that all cells in the regeneration blastema are randomly distributed throughout the cell cycle and actively cycling towards the next mitotic division. We now show that data from continuous labeling (3H-thymidine) experiments do not support this view and favor instead the hypothesis that the blastema cell cycle is punctuated in the g1 phase wherein cells can enter what we term a transiently quiescent (TQ) position. We call this hypothesis the punctuated cycling (PC) hypothesis. We further propose that the relative sizes of the quiescent and actively cycling populations explain (1) variations in rates of regeneration in different sizes of urodele amphibians, (2) the rate and success of regeneration in different species, and (3) how various controlling factors, such as injury, nerves, growth factors, wound epidermis, and hormones, influence the initiation and progression of the regeneration process. This PC hypothesis is important for interpreting previous pulse labeling data, is consistent with recently obtained continuous labeling data, and is experimentally testable.
UR - http://www.scopus.com/inward/record.url?scp=0021987430&partnerID=8YFLogxK
U2 - 10.1111/j.1432-0436.1985.tb00824.x
DO - 10.1111/j.1432-0436.1985.tb00824.x
M3 - Article
AN - SCOPUS:0021987430
VL - 28
SP - 195
EP - 199
JO - Differentiation
JF - Differentiation
SN - 0301-4681
IS - 3
ER -