Pulmonary vasculature directed adenovirus increases epithelial lining fluid alpha-1 antitrypsin levels

Maurizio Buggio, Christopher Towe, Anand Annan, Sergey Kaliberov, Zhi Hong Lu, Calvin Stephens, Jeffrey M. Arbeit, David T. Curiel

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Background: Gene therapy for inherited serum deficiency disorders has previously been limited by the balance between obtaining adequate expression and causing hepatic toxicity. Our group has previously described modifications of a replication deficient human adenovirus serotype 5 that increase pulmonary vasculature transgene expression. Methods: In the present study, we use a modified pulmonary targeted adenovirus to express human alpha-1 antitrypsin (A1AT) in C57BL/6J mice. Results: Using the targeted adenovirus, we were able to achieve similar increases in serum A1AT levels with less liver viral uptake. We also increased pulmonary epithelial lining fluid A1AT levels by more than an order of magnitude compared to that of untargeted adenovirus expressing A1AT in a mouse model. These gains are achieved along with evidence of decreased systemic inflammation and no evidence for increased inflammation within the vector-targeted end organ. Conclusions: In addition to comprising a step towards clinically viable gene therapy for A1AT, maximization of protein production at the site of action represents a significant technical advancement in the field of systemically delivered pulmonary targeted gene therapy. It also provides an alternative to the previous limitations of hepatic viral transduction and associated toxicities.

Original languageEnglish
Pages (from-to)38-44
Number of pages7
JournalJournal of Gene Medicine
Volume18
Issue number1-3
DOIs
StatePublished - Jan 1 2016

Keywords

  • Adenovirus
  • Alpha-1 antitrypsin
  • Gene therapy

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