Pulmonary surfactant proteins A and D enhance neutrophil uptake of bacteria

Kevan L. Hartshorn, Erika Crouch, Mitchell R. White, Maria L. Colamussi, Anand Kakkanatt, Benjamin Tauber, Virginia Shepherd, Kedarnath N. Sastry

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199 Scopus citations

Abstract

The collectins are a class of collagenous lectin proteins present in serum and pulmonary secretions [pulmonary surfactant protein (SP) A and SP- D] that are believed to participate in innate immune responses to various pathogens. With the use of flow cytometric and fluorescent-microscopic assays, SP-A and SP-D were shown to increase calcium-dependent neutrophil uptake of Escherichia coli, Streptococcus pneumoniae, and Staphylococcus aureus. Evidence is provided that the collectins enhanced bacterial uptake through a mechanism that involved both bacterial aggregation and direct actions on neutrophils. The degree of multimerization of SP-D preparations was a critical determinant of both aggregating activity and potency in enhancing bacterial uptake. The mechanisms of opsonizing activity of SP-D and SP-A differed in important respects from those of opsonizing antibodies. These results provide the first evidence that surfactant collectins may promote neutrophil-mediated clearance of bacteria in the lung independently of opsonizing antibody.

Original languageEnglish
Pages (from-to)L958-L969
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume274
Issue number6 18-6
DOIs
StatePublished - Jun 1998

Keywords

  • Antibodies
  • Collectins
  • Escherichia coli
  • Opsonization
  • Streptococcus pneumoniae

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    Hartshorn, K. L., Crouch, E., White, M. R., Colamussi, M. L., Kakkanatt, A., Tauber, B., Shepherd, V., & Sastry, K. N. (1998). Pulmonary surfactant proteins A and D enhance neutrophil uptake of bacteria. American Journal of Physiology - Lung Cellular and Molecular Physiology, 274(6 18-6), L958-L969. https://doi.org/10.1152/ajplung.1998.274.6.l958