PTEN mutations in endometrial cancers with 10q LOH: Additional evidence for the involvement of multiple tumor suppressors

Sally B. Simpkins, Stacia Peiffer-Schneider, David G. Mutch, Deborah Gersell, Paul J. Goodfellow

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

Objective. The aim of this study was to assess the involvement of PTEN and other putative 10q tumor suppressors in endometrioid-type adenocarcinomas characterized by loss of 10q sequences. Methods. PCR-based single-stranded conformational variant analysis and sequencing of individual PTEN exons in 34 tumor specimens and their corresponding normal DNA were used. Results. Thirteen of the 34 tumors (38%) revealed a PTEN mutation: 2 frameshift, 3 nonsense, 3 missense, 3 splice site alterations, and 2 homozygous deletions. Conclusion. The observation that greater than 60% of endometrial cancers with 10q LOH lack PTEN mutations, in addition to previously reported LOH data, provides evidence for the existence of other tumor suppressors on 10q. Consideration of PTEN mutation status may prove important in deletion mapping studies to locate additional tumor suppressors on the long arm of chromosome 10.

Original languageEnglish
Pages (from-to)391-395
Number of pages5
JournalGynecologic oncology
Volume71
Issue number3
DOIs
StatePublished - Dec 1998

Keywords

  • Endometrial cancer
  • LOH
  • PTEN
  • SSCV
  • Tumor suppressor gene

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