Ptc7p Dephosphorylates Select Mitochondrial Proteins to Enhance Metabolic Function

Xiao Guo, Natalie M. Niemi, Paul D. Hutchins, Samson G.F. Condon, Adam Jochem, Arne Ulbrich, Alan J. Higbee, Jason D. Russell, Alessandro Senes, Joshua J. Coon, David J. Pagliarini

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Proper maintenance of mitochondrial activity is essential for metabolic homeostasis. Widespread phosphorylation of mitochondrial proteins may be an important element of this process; yet, little is known about which enzymes control mitochondrial phosphorylation or which phosphosites have functional impact. We investigate these issues by disrupting Ptc7p, a conserved but largely uncharacterized mitochondrial matrix PP2C-type phosphatase. Loss of Ptc7p causes respiratory growth defects concomitant with elevated phosphorylation of select matrix proteins. Among these, Δptc7 yeast exhibit an increase in phosphorylation of Cit1p, the canonical citrate synthase of the tricarboxylic acid (TCA) cycle, that diminishes its activity. We find that phosphorylation of S462 can eliminate Cit1p enzymatic activity likely by disrupting its proper dimerization, and that Ptc7p-driven dephosphorylation rescues Cit1p activity. Collectively, our work connects Ptc7p to an essential TCA cycle function and to additional phosphorylation events that may affect mitochondrial activity inadvertently or in a regulatory manner.

Original languageEnglish
Pages (from-to)307-313
Number of pages7
JournalCell Reports
Volume18
Issue number2
DOIs
StatePublished - Jan 10 2017

Keywords

  • Pptc7
  • Ptc7
  • citrate synthase
  • mitochondria
  • mitochondrial phosphorylation
  • phosphatase
  • phosphoproteomics

Fingerprint

Dive into the research topics of 'Ptc7p Dephosphorylates Select Mitochondrial Proteins to Enhance Metabolic Function'. Together they form a unique fingerprint.

Cite this