TY - JOUR
T1 - pseudomonas aeruginosa corneal ulcer isolates distinguished using the arbitrarily primed PCR DNA fingerprinting method
AU - Bukanov, Nickolai
AU - Ravi, V. Nathan
AU - Miller, Darlene
AU - Srivastava, Kalpana
AU - Berg, Douglas E.
N1 - Funding Information:
This work was supported by grant HG00563 and DK48029 from the US Public Health Service to DEB, and a grant from the Society for the Prevention of Blindness to the Department of Ophthamology, Washington University Medical School.
PY - 1994
Y1 - 1994
N2 - Infection of the eye by Pseudomonas aeruginosa can result in corneal inflammation (keratitis) and ulceration, and permanent decrease in vision if not successfully treated. We tested for diversity among P. aeruginosa strains from corneal ulcers by the sensitive and efficient 'RAPD' (for 'random amplified polymorphic DNA' fingerprinting method. This method uses single oligonucleotides of arbitrarily chosen sequence as primers in low-stringency PCR amplification, and results in strain-specific arrays of DNA fragments. Tests of 20 independent P. aeruginosa corneal ulcer isolates yielded 19 different arrays of products with each of three arbitrary primers, indicating that all but two of the strains differed from one another. Additional isolates from three patients (infected eye, contact lens or eye drops) yielded fragment patterns that were identical to those of the original isolate in each case. Thus, our results demonstrate considerable diversity among P. aeruginosa corneal ulcer isolates, and suggest that just one clone may predominate in typical infections.
AB - Infection of the eye by Pseudomonas aeruginosa can result in corneal inflammation (keratitis) and ulceration, and permanent decrease in vision if not successfully treated. We tested for diversity among P. aeruginosa strains from corneal ulcers by the sensitive and efficient 'RAPD' (for 'random amplified polymorphic DNA' fingerprinting method. This method uses single oligonucleotides of arbitrarily chosen sequence as primers in low-stringency PCR amplification, and results in strain-specific arrays of DNA fragments. Tests of 20 independent P. aeruginosa corneal ulcer isolates yielded 19 different arrays of products with each of three arbitrary primers, indicating that all but two of the strains differed from one another. Additional isolates from three patients (infected eye, contact lens or eye drops) yielded fragment patterns that were identical to those of the original isolate in each case. Thus, our results demonstrate considerable diversity among P. aeruginosa corneal ulcer isolates, and suggest that just one clone may predominate in typical infections.
KW - Arbitrarily primed polymerase chain reaction (PCR)
KW - Corneal ulcers
KW - Human
KW - Keratitis
KW - Pseudomonas aeruginosa
KW - Random amplified polymorphic DNA (RAPD) fingerprinting
UR - http://www.scopus.com/inward/record.url?scp=0028116435&partnerID=8YFLogxK
U2 - 10.3109/02713689409025132
DO - 10.3109/02713689409025132
M3 - Article
C2 - 7851113
AN - SCOPUS:0028116435
SN - 0271-3683
VL - 13
SP - 783
EP - 790
JO - Current Eye Research
JF - Current Eye Research
IS - 11
ER -