TY - JOUR
T1 - PRP-17 and the pre-mRNA splicing pathway are preferentially required for the proliferation versus meiotic development decision and germline sex determination in Caenorhabditis elegans
AU - Kerins, Jessica Amrozowicz
AU - Hanazawa, Momoyo
AU - Dorsett, Maia
AU - Schedl, Tim
PY - 2010/5
Y1 - 2010/5
N2 - In C. elegans, the decision between germline stem cell proliferation and entry into meiosis is controlled by GLP-1 Notch signaling, which promotes proliferation through repression of the redundant GLD-1 and GLD-2 pathways that direct meiotic entry. We identify prp-17 as another gene functioning downstream of GLP-1 signaling that promotes meiotic entry, largely by acting on the GLD-1 pathway, and that also functions in female germline sex determination. PRP-17 is orthologous to the yeast and human pre-mRNA splicing factor PRP17/CDC40 and can rescue the temperature-sensitive lethality of yeast PRP17. This link to splicing led to an RNAi screen of predicted C. elegans splicing factors in sensitized genetic backgrounds. We found that many genes throughout the splicing cascade function in the proliferation/meiotic entry decision and germline sex determination indicating that splicing per se, rather than a novel function of a subset of splicing factors, is necessary for these processes.
AB - In C. elegans, the decision between germline stem cell proliferation and entry into meiosis is controlled by GLP-1 Notch signaling, which promotes proliferation through repression of the redundant GLD-1 and GLD-2 pathways that direct meiotic entry. We identify prp-17 as another gene functioning downstream of GLP-1 signaling that promotes meiotic entry, largely by acting on the GLD-1 pathway, and that also functions in female germline sex determination. PRP-17 is orthologous to the yeast and human pre-mRNA splicing factor PRP17/CDC40 and can rescue the temperature-sensitive lethality of yeast PRP17. This link to splicing led to an RNAi screen of predicted C. elegans splicing factors in sensitized genetic backgrounds. We found that many genes throughout the splicing cascade function in the proliferation/meiotic entry decision and germline sex determination indicating that splicing per se, rather than a novel function of a subset of splicing factors, is necessary for these processes.
KW - GLP-1 notch signaling
KW - Germline development
KW - Germline sex determination
KW - Meiotic entry
KW - PRP-17
KW - Pre-mRNA splicing
KW - Proliferation
UR - http://www.scopus.com/inward/record.url?scp=77951536653&partnerID=8YFLogxK
U2 - 10.1002/dvdy.22274
DO - 10.1002/dvdy.22274
M3 - Article
C2 - 20419786
AN - SCOPUS:77951536653
VL - 239
SP - 1555
EP - 1572
JO - Developmental Dynamics
JF - Developmental Dynamics
SN - 1058-8388
IS - 5
ER -