TY - JOUR
T1 - Protocol to isolate RBP-mRNA complexes using RNA-CLIP and examine target mRNAs
AU - Blanc, Valerie
AU - Molitor, Elizabeth A.
AU - Davidson, Nicholas O.
N1 - Publisher Copyright:
© 2023 The Authors
PY - 2023/6/16
Y1 - 2023/6/16
N2 - RNA-binding proteins (RBPs) regulate diverse functions by interacting with target transcripts. Here we present a protocol to isolate RBP-mRNA complexes using RNA-CLIP and examine target mRNAs in association with ribosomal populations. We describe steps to identify specific RBPs and RNA targets reflecting a variety of developmental, physiological, and pathological states. This protocol enables RNP complex isolation from tissue sources (liver and small intestine) or populations of primary cells (hepatocytes), but not at a single-cell level. For complete details on the use and execution of this protocol, please refer to Blanc et al. (2014)1 and Blanc et al. (2021).2
AB - RNA-binding proteins (RBPs) regulate diverse functions by interacting with target transcripts. Here we present a protocol to isolate RBP-mRNA complexes using RNA-CLIP and examine target mRNAs in association with ribosomal populations. We describe steps to identify specific RBPs and RNA targets reflecting a variety of developmental, physiological, and pathological states. This protocol enables RNP complex isolation from tissue sources (liver and small intestine) or populations of primary cells (hepatocytes), but not at a single-cell level. For complete details on the use and execution of this protocol, please refer to Blanc et al. (2014)1 and Blanc et al. (2021).2
KW - Cell Separation/Fractionation
KW - Gene Expression
KW - RNAseq
UR - http://www.scopus.com/inward/record.url?scp=85160106895&partnerID=8YFLogxK
U2 - 10.1016/j.xpro.2023.102313
DO - 10.1016/j.xpro.2023.102313
M3 - Article
C2 - 37220002
AN - SCOPUS:85160106895
SN - 2666-1667
VL - 4
JO - STAR Protocols
JF - STAR Protocols
IS - 2
M1 - 102313
ER -