Abstract
Cellular RNAs are modified by both physiological factors and exogenous agents, such as methyl methanesulfonate (MMS). However, techniques for analyzing how proteins may interact with these modified RNAs are limited. Here, we provide a protocol combining RNA immunoprecipitation (RIP) with mass spectrometry (MS) to analyze the methylation state of the RNAs bound by Flag-tagged proteins in mammalian cells. The approach is highly quantitative and can simultaneously detect several methylated nucleosides in a single experiment. For complete details on the use and execution of this protocol, please refer to Tsao et al. (2021).
Original language | English |
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Article number | 101268 |
Journal | STAR Protocols |
Volume | 3 |
Issue number | 2 |
DOIs | |
State | Published - Jun 17 2022 |
Keywords
- Mass Spectrometry
- Molecular Biology
- Protein Biochemistry
- Proteomics