Abstract
Tumors originating from the head and neck represent diverse histologies and are comprised of several cell types, including malignant cells, cancer-associated fibroblasts, endothelial cells, and immune cells. In this protocol, we describe a step-by-step approach for the dissociation of fresh human head and neck tumor specimens, followed by isolation of viable single cells using fluorescence-activated cell sorting. Our protocol facilitates the effective downstream use of techniques, including single-cell RNA sequencing and generation of three-dimensional patient-derived organoids. For complete details on the use and execution of this protocol, please refer to Puram et al. (2017)1 and Parikh et al. (2022).2
Original language | English |
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Article number | 102294 |
Journal | STAR Protocols |
Volume | 4 |
Issue number | 2 |
DOIs | |
State | Published - Jun 16 2023 |
Keywords
- Cell Biology
- Cell culture
- Cell isolation
- Cell separation/fractionation
- Flow Cytometry/Mass Cytometry
- Single Cell