Protocol for tumor dissociation and fluorescence-activated cell sorting of human head and neck cancers

Ogoegbunam Okolo, Victoria Yu, Samuel Flashner, Cecilia Martin, Hiroshi Nakagawa, Derrick T. Lin, Sidharth V. Puram, Anuraag S. Parikh

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Tumors originating from the head and neck represent diverse histologies and are comprised of several cell types, including malignant cells, cancer-associated fibroblasts, endothelial cells, and immune cells. In this protocol, we describe a step-by-step approach for the dissociation of fresh human head and neck tumor specimens, followed by isolation of viable single cells using fluorescence-activated cell sorting. Our protocol facilitates the effective downstream use of techniques, including single-cell RNA sequencing and generation of three-dimensional patient-derived organoids. For complete details on the use and execution of this protocol, please refer to Puram et al. (2017)1 and Parikh et al. (2022).2

Original languageEnglish
Article number102294
JournalSTAR Protocols
Volume4
Issue number2
DOIs
StatePublished - Jun 16 2023

Keywords

  • Cell Biology
  • Cell culture
  • Cell isolation
  • Cell separation/fractionation
  • Flow Cytometry/Mass Cytometry
  • Single Cell

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