Protocol for a non-surgical model of perineural invasion for assessing neural drivers of cancer aggressiveness in mice

Tiago H. Zaninelli; Telma Saraiva-Santos; Brielle H. Patlin; Michael Slade Stratton; David Y. Chen; Felipe A. Pinho-Ribeiro

doi:10.1016/j.xpro.2024.103345

Protocol for a non-surgical model of perineural invasion for assessing neural drivers of cancer aggressiveness in mice

Tiago H. Zaninelli, Telma Saraiva-Santos, Brielle H. Patlin, Michael Slade Stratton, David Y. Chen, Felipe A. Pinho-Ribeiro

Research output: Contribution to journal › Article › peer-review

Abstract

Perineural invasion (PNI) is a significant risk factor for cancer recurrence and metastasis; however, its mechanisms relating to cancer aggressiveness remain poorly understood. Here, we present a protocol for a non-surgical model of PNI in mice using a neurotropic melanoma cell line that migrates from the skin to the sciatic nerve. We describe the steps for cell culture and injection, tumor burden measurements, mouse euthanasia, and tissue dissection. We then detail procedures for sample cross-section and confocal imaging.

Original language	English
Article number	103345
Journal	STAR Protocols
Volume	5
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2024.103345
State	Published - Dec 20 2024

Keywords

Cancer
Model Organisms
Neuroscience

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10.1016/j.xpro.2024.103345

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title = "Protocol for a non-surgical model of perineural invasion for assessing neural drivers of cancer aggressiveness in mice",

abstract = "Perineural invasion (PNI) is a significant risk factor for cancer recurrence and metastasis; however, its mechanisms relating to cancer aggressiveness remain poorly understood. Here, we present a protocol for a non-surgical model of PNI in mice using a neurotropic melanoma cell line that migrates from the skin to the sciatic nerve. We describe the steps for cell culture and injection, tumor burden measurements, mouse euthanasia, and tissue dissection. We then detail procedures for sample cross-section and confocal imaging.",

keywords = "Cancer, Model Organisms, Neuroscience",

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AU - Zaninelli, Tiago H.

AU - Saraiva-Santos, Telma

AU - Patlin, Brielle H.

AU - Stratton, Michael Slade

AU - Chen, David Y.

AU - Pinho-Ribeiro, Felipe A.

PY - 2024/12/20

Y1 - 2024/12/20

N2 - Perineural invasion (PNI) is a significant risk factor for cancer recurrence and metastasis; however, its mechanisms relating to cancer aggressiveness remain poorly understood. Here, we present a protocol for a non-surgical model of PNI in mice using a neurotropic melanoma cell line that migrates from the skin to the sciatic nerve. We describe the steps for cell culture and injection, tumor burden measurements, mouse euthanasia, and tissue dissection. We then detail procedures for sample cross-section and confocal imaging.

AB - Perineural invasion (PNI) is a significant risk factor for cancer recurrence and metastasis; however, its mechanisms relating to cancer aggressiveness remain poorly understood. Here, we present a protocol for a non-surgical model of PNI in mice using a neurotropic melanoma cell line that migrates from the skin to the sciatic nerve. We describe the steps for cell culture and injection, tumor burden measurements, mouse euthanasia, and tissue dissection. We then detail procedures for sample cross-section and confocal imaging.

KW - Cancer

KW - Model Organisms

KW - Neuroscience

UR - http://www.scopus.com/inward/record.url?scp=85207797898&partnerID=8YFLogxK

U2 - 10.1016/j.xpro.2024.103345

DO - 10.1016/j.xpro.2024.103345

M3 - Article

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AN - SCOPUS:85207797898

SN - 2666-1667

VL - 5

JO - STAR Protocols

JF - STAR Protocols

IS - 4

M1 - 103345

ER -

Protocol for a non-surgical model of perineural invasion for assessing neural drivers of cancer aggressiveness in mice

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Keywords

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