TY - JOUR
T1 - Proteomics-based expression library screening (PELS)
AU - Kudva, Indira T.
AU - Krastins, Bryan
AU - Sheng, Haiqing
AU - Griffin, Robert W.
AU - Sarracino, David A.
AU - Tarr, Phillip I.
AU - Hovde, Carolyn J.
AU - Calderwood, Stephen B.
AU - John, Manohar
PY - 2006/8
Y1 - 2006/8
N2 - Current methodologies for global identification of microbial proteins that elicit host humoral immune responses have several limitations and are not ideally suited for use in the postgenomic era. Here we describe a novel application of proteomics, proteomics-based expression library screening, to rapidly define microbial immunoproteomes. Proteomics-based expression library screening is broadly applicable to any cultivable, sequenced pathogen eliciting host antibody responses and hence is ideal for rapidly mining microbial proteomes for targets with diagnostic, prophylactic, and therapeutic potential. In this report, we demonstrate "proof-of-principle" by identifying 207 proteins of the Escherichia coli O157:H7 immunome in bovine reservoirs in only 3 weeks.
AB - Current methodologies for global identification of microbial proteins that elicit host humoral immune responses have several limitations and are not ideally suited for use in the postgenomic era. Here we describe a novel application of proteomics, proteomics-based expression library screening, to rapidly define microbial immunoproteomes. Proteomics-based expression library screening is broadly applicable to any cultivable, sequenced pathogen eliciting host antibody responses and hence is ideal for rapidly mining microbial proteomes for targets with diagnostic, prophylactic, and therapeutic potential. In this report, we demonstrate "proof-of-principle" by identifying 207 proteins of the Escherichia coli O157:H7 immunome in bovine reservoirs in only 3 weeks.
UR - http://www.scopus.com/inward/record.url?scp=33748302259&partnerID=8YFLogxK
U2 - 10.1074/mcp.T600013-MCP200
DO - 10.1074/mcp.T600013-MCP200
M3 - Article
C2 - 16737953
AN - SCOPUS:33748302259
SN - 1535-9476
VL - 5
SP - 1514
EP - 1519
JO - Molecular and Cellular Proteomics
JF - Molecular and Cellular Proteomics
IS - 8
ER -