Proteomics-based expression library screening (PELS)

Indira T. Kudva, Bryan Krastins, Haiqing Sheng, Robert W. Griffin, David A. Sarracino, Phillip I. Tarr, Carolyn J. Hovde, Stephen B. Calderwood, Manohar John

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Current methodologies for global identification of microbial proteins that elicit host humoral immune responses have several limitations and are not ideally suited for use in the postgenomic era. Here we describe a novel application of proteomics, proteomics-based expression library screening, to rapidly define microbial immunoproteomes. Proteomics-based expression library screening is broadly applicable to any cultivable, sequenced pathogen eliciting host antibody responses and hence is ideal for rapidly mining microbial proteomes for targets with diagnostic, prophylactic, and therapeutic potential. In this report, we demonstrate "proof-of-principle" by identifying 207 proteins of the Escherichia coli O157:H7 immunome in bovine reservoirs in only 3 weeks.

Original languageEnglish
Pages (from-to)1514-1519
Number of pages6
JournalMolecular and Cellular Proteomics
Volume5
Issue number8
DOIs
StatePublished - Aug 2006

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    Kudva, I. T., Krastins, B., Sheng, H., Griffin, R. W., Sarracino, D. A., Tarr, P. I., Hovde, C. J., Calderwood, S. B., & John, M. (2006). Proteomics-based expression library screening (PELS). Molecular and Cellular Proteomics, 5(8), 1514-1519. https://doi.org/10.1074/mcp.T600013-MCP200