Proteomic and phylogenetic coevolution analyses of pM79 and pM92 identify interactions with RNA polymerase II and delineate the murine cytomegalovirus late transcription complex

Travis J. Chapa, Yushen Du, Ren Sun, Dong Yu, Anthony R. French

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The regulation of the late viral gene expression in betaherpesviruses is largely undefined. We have previously shown that the murine cytomegalovirus proteins pM79 and pM92 are required for late gene transcription. Here, we provide insight into the mechanism of pM79 and pM92 activity by determining their interaction partners during infection. Co-immunoprecipitationcoupled MS studies demonstrate that pM79 and pM92 interact with an array of cellular and viral proteins involved in transcription. Specifically, we identify RNA polymerase II as a cellular target for both pM79 and pM92. We use inter-protein coevolution analysis to show how pM79 and pM92 likely assemble into a late transcription complex composed of late transcription regulators pM49, pM87 and pM95. Combining proteomic methods with coevolution computational analysis provides novel insights into the relationship between pM79, pM92 and RNA polymerase II and allows the generation of a model of the multi-component viral complex that regulates late gene transcription.

Original languageEnglish
Pages (from-to)242-250
Number of pages9
JournalJournal of General Virology
Volume98
Issue number2
DOIs
StatePublished - Feb 2017

Keywords

  • Coevolution
  • Late viral transcription
  • MCMV
  • Proteomics

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