Proteomic analysis reveals GIT1 as a novel mTOR complex component critical for mediating astrocyte survival

Laura J. Smithson, David H. Gutmann

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

As a critical regulator of cell growth, the mechanistic target of rapamycin (mTOR) protein operates as part of two molecularly and functionally distinct complexes. Herein, we demonstrate that mTOR complex molecular composition varies in different somatic tissues. In astrocytes and neural stem cells, we identified G-protein-coupled receptor kinase-interacting protein 1 (GIT1) as a novel mTOR-binding protein, creating a unique mTOR complex lacking Raptor and Rictor. Moreover, GIT1 binding to mTOR is regulated by AKT activation and is essential for mTOR-mediated astrocyte survival. Together, these data reveal that mTOR complex function is partly dictated by its molecuflar composition in different cell types.

Original languageEnglish
Pages (from-to)1383-1388
Number of pages6
JournalGenes and Development
Volume30
Issue number12
DOIs
StatePublished - Jun 15 2016

Keywords

  • Astrocytes
  • Brain
  • GIT1
  • MTOR

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