TY - JOUR
T1 - Proteomic analysis of the SIRT6 interactome
T2 - Novel links to genome maintenance and cellular stress signaling
AU - Simeoni, Federica
AU - Tasselli, Luisa
AU - Tanaka, Shinji
AU - Villanova, Lidia
AU - Hayashi, Mayumi
AU - Kubota, Kazuishi
AU - Isono, Fujio
AU - Garcia, Benjamin A.
AU - Michishita-Kioi, Eriko
AU - Chua, Katrin F.
N1 - Funding Information:
We thank Scott Carlson, Silvana Paredes, Or Gozani, Jerome Bonnet and members of the Chua laboratories for technical advice and discussions. This work was supported by grants from the National Institutes of Health (NIH) to K.F.C. (R01 AG028867), the Department of Veterans Affairs to K.F.C. (Merit Award); and by fellowship awards to F.S. (McCormick Award), L.T. (American Italian Cancer Foundation Post-doctoral Research Fellowship), and L.V. (Fondation of Italian Research for Cancer). K.F.C. was a Paul Beeson Scholar and an Ellison Medical Foundation New Scholar in Aging.
Funding Information:
Supplementary information accompanies this paper at http://www.nature.com/ scientificreports Competing financial interests: Dr. Chua’s research is partly funded by Daiichi Sankyo company. Dr. Michishita-Kioi, Mr. Tanaka, Dr. Isono, Ms. Hayashi, and Dr. Kubota are employees of Daiichi Sankyo Group Companies.
PY - 2013
Y1 - 2013
N2 - The chromatin regulatory factor SIRT6 plays pivotal roles in metabolism, tumor suppression, and aging biology. Despite the fundamental roles of SIRT6 in physiology and disease, only a handful of molecular and functional interactions of SIRT6 have been reported. Here, we characterize the SIRT6 interactome and identify 80+ novel SIRT6-interacting proteins. The discovery of these SIRT6-associations considerably expands knowledge of the SIRT6 interaction network, and suggests previously unknown functional interactions of SIRT6 in fundamental cellular processes. These include chromatin remodeling, mitotic chromosome segregation, protein homeostasis, and transcriptional elongation. Extended analysis of the SIRT6 interaction with G3BP1, a master stress response factor, uncovers an unexpected role and mechanism of SIRT6 in regulating stress granule assembly and cellular stress resistance.
AB - The chromatin regulatory factor SIRT6 plays pivotal roles in metabolism, tumor suppression, and aging biology. Despite the fundamental roles of SIRT6 in physiology and disease, only a handful of molecular and functional interactions of SIRT6 have been reported. Here, we characterize the SIRT6 interactome and identify 80+ novel SIRT6-interacting proteins. The discovery of these SIRT6-associations considerably expands knowledge of the SIRT6 interaction network, and suggests previously unknown functional interactions of SIRT6 in fundamental cellular processes. These include chromatin remodeling, mitotic chromosome segregation, protein homeostasis, and transcriptional elongation. Extended analysis of the SIRT6 interaction with G3BP1, a master stress response factor, uncovers an unexpected role and mechanism of SIRT6 in regulating stress granule assembly and cellular stress resistance.
UR - http://www.scopus.com/inward/record.url?scp=84886995483&partnerID=8YFLogxK
U2 - 10.1038/srep03085
DO - 10.1038/srep03085
M3 - Article
C2 - 24169447
AN - SCOPUS:84886995483
SN - 2045-2322
VL - 3
JO - Scientific reports
JF - Scientific reports
M1 - 3085
ER -