TY - JOUR
T1 - Proteomic analysis of gastric cancer and immunoblot validation of potential biomarkers
AU - Kočevar, Nina
AU - Odreman, Federico
AU - Vindigni, Alessandro
AU - Grazio, Snjěana Frković
AU - Komel, Radovan
PY - 2012
Y1 - 2012
N2 - AIM: To search for and validate differentially expressed proteins in patients with gastric adenocarcinoma. METHODS: We used two-dimensional gel electrophoresis and mass spectrometry to search for differentially expressed proteins in patients with gastric adenocarcinoma. A set of proteins was validated with immunoblotting. RESULTS: We identified 30 different proteins involved in various biological processes: metabolism, development, death, response to stress, cell cycle, cell communication, transport, and cell motility. Eight proteins were chosen for further validation by immunoblotting. Our results show that gastrokine-1, 39S ribosomal protein L12 (mitochondrial precursor), plasma cell-induced resident endoplasmic reticulum protein, and glutathione S-transferase mu 3 were significantly underexpressed in gastric adenocarcinoma relative to adjacent non-tumor tissue samples. On the other hand, septin-2, ubiquitin-conjugating enzyme E2 N, and transaldolase were significantly overexpressed. Translationally controlled tumor protein was shown to be differentially expressed only in patients with cancer of the gastric cardia/esophageal border.CONCLUSION: This work presents a set of possible diagnostic biomarkers, validated for the first time. It might contribute to the efforts of understanding gastric cancer carcinogenesis.
AB - AIM: To search for and validate differentially expressed proteins in patients with gastric adenocarcinoma. METHODS: We used two-dimensional gel electrophoresis and mass spectrometry to search for differentially expressed proteins in patients with gastric adenocarcinoma. A set of proteins was validated with immunoblotting. RESULTS: We identified 30 different proteins involved in various biological processes: metabolism, development, death, response to stress, cell cycle, cell communication, transport, and cell motility. Eight proteins were chosen for further validation by immunoblotting. Our results show that gastrokine-1, 39S ribosomal protein L12 (mitochondrial precursor), plasma cell-induced resident endoplasmic reticulum protein, and glutathione S-transferase mu 3 were significantly underexpressed in gastric adenocarcinoma relative to adjacent non-tumor tissue samples. On the other hand, septin-2, ubiquitin-conjugating enzyme E2 N, and transaldolase were significantly overexpressed. Translationally controlled tumor protein was shown to be differentially expressed only in patients with cancer of the gastric cardia/esophageal border.CONCLUSION: This work presents a set of possible diagnostic biomarkers, validated for the first time. It might contribute to the efforts of understanding gastric cancer carcinogenesis.
KW - Biomarkers
KW - Gastric adenocarcinoma
KW - Mass spectrometry
KW - Proteome
KW - Two-dimensional gel electrophoresis
UR - http://www.scopus.com/inward/record.url?scp=84858778062&partnerID=8YFLogxK
U2 - 10.3748/wjg.v18.i11.1216
DO - 10.3748/wjg.v18.i11.1216
M3 - Article
C2 - 22468085
AN - SCOPUS:84858778062
SN - 1007-9327
VL - 18
SP - 1216
EP - 1228
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
IS - 11
ER -