Proteomic analysis of an extreme halophilic archaeon Halobacterium species NRC-1

Young Ah Goo; Eugene C. Yi; W. Andy Tao; Nitin S. Baliga; Min Pan; David R. Goodlett; Lee Hood; Wailap V. Ng

Proteomic analysis of an extreme halophilic archaeon Halobacterium species NRC-1

Young Ah Goo, Eugene C. Yi, W. Andy Tao, Nitin S. Baliga, Min Pan, David R. Goodlett, Lee Hood, Wailap V. Ng

Research output: Contribution to conference › Paper › peer-review

Abstract

The proteins expressed by extreme halophilic archaeon Halobacterium species NRC-1 were identified. Soluble and membrane proteins produced by the organism were collected in different fractions according to their solubility, were denatured, reduced, and finally subjected to tryptic digestion. Mass spectra were then acquired by data-dependent ion selection form a full range as well as discrete narrow survey scan m/z ranges to increase the number of identifications. It was found that a total of 442 proteins had one or more peptides with highly distinguishable MS/MS spectra and relative low noise.

Original language	English
Pages	221-222
Number of pages	2
State	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	06/2/02 → 06/6/02

Cite this

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title = "Proteomic analysis of an extreme halophilic archaeon Halobacterium species NRC-1",

abstract = "The proteins expressed by extreme halophilic archaeon Halobacterium species NRC-1 were identified. Soluble and membrane proteins produced by the organism were collected in different fractions according to their solubility, were denatured, reduced, and finally subjected to tryptic digestion. Mass spectra were then acquired by data-dependent ion selection form a full range as well as discrete narrow survey scan m/z ranges to increase the number of identifications. It was found that a total of 442 proteins had one or more peptides with highly distinguishable MS/MS spectra and relative low noise.",

author = "Goo, {Young Ah} and Yi, {Eugene C.} and Tao, {W. Andy} and Baliga, {Nitin S.} and Min Pan and Goodlett, {David R.} and Lee Hood and Ng, {Wailap V.}",

year = "2002",

language = "English",

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note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

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T1 - Proteomic analysis of an extreme halophilic archaeon Halobacterium species NRC-1

AU - Goo, Young Ah

AU - Yi, Eugene C.

AU - Tao, W. Andy

AU - Baliga, Nitin S.

AU - Pan, Min

AU - Goodlett, David R.

AU - Hood, Lee

AU - Ng, Wailap V.

PY - 2002

Y1 - 2002

N2 - The proteins expressed by extreme halophilic archaeon Halobacterium species NRC-1 were identified. Soluble and membrane proteins produced by the organism were collected in different fractions according to their solubility, were denatured, reduced, and finally subjected to tryptic digestion. Mass spectra were then acquired by data-dependent ion selection form a full range as well as discrete narrow survey scan m/z ranges to increase the number of identifications. It was found that a total of 442 proteins had one or more peptides with highly distinguishable MS/MS spectra and relative low noise.

AB - The proteins expressed by extreme halophilic archaeon Halobacterium species NRC-1 were identified. Soluble and membrane proteins produced by the organism were collected in different fractions according to their solubility, were denatured, reduced, and finally subjected to tryptic digestion. Mass spectra were then acquired by data-dependent ion selection form a full range as well as discrete narrow survey scan m/z ranges to increase the number of identifications. It was found that a total of 442 proteins had one or more peptides with highly distinguishable MS/MS spectra and relative low noise.

UR - http://www.scopus.com/inward/record.url?scp=2442699182&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442699182

SP - 221

EP - 222

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Proteomic analysis of an extreme halophilic archaeon Halobacterium species NRC-1

Abstract

Conference

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