Proteolytic surface functionalization enhances in vitro magnetic nanoparticle mobility through extracellular matrix

Sam J. Kuhn; Stephanie K. Finch; Dennis E. Hallahan; Todd D. Giorgio

doi:10.1021/nl052241g

Proteolytic surface functionalization enhances in vitro magnetic nanoparticle mobility through extracellular matrix

Sam J. Kuhn, Stephanie K. Finch, Dennis E. Hallahan, Todd D. Giorgio

Research output: Contribution to journal › Article › peer-review

84 Scopus citations

Abstract

Steric barriers such as collagen I sharply limit Interstitial delivery of macromolecular and nanoparticle (NP) based therapeutic agents. Collagenase-linked superparamagnetic NPs overcame these barriers and moved through in vitro extracellular matrix (ECM) at 90 μm h ^-1, a rate similar to invasive cells, under the influence of a magnetic field. NP migration in ECM diminished linearly over 5 days. The collagenase-NP construct overcame two of the most significant barriers to nano- and mlcroscale therapeutics deployment: proteolytic enzyme stability was maintained during a clinically useful time frame by immobilization on the NP surface and degradation of interstitial barriers to tissue biodistribution was enabled by the conjugated microbial protease.

Original language	English
Pages (from-to)	306-312
Number of pages	7
Journal	Nano Letters
Volume	6
Issue number	2
DOIs	https://doi.org/10.1021/nl052241g
State	Published - Feb 2006

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title = "Proteolytic surface functionalization enhances in vitro magnetic nanoparticle mobility through extracellular matrix",

abstract = "Steric barriers such as collagen I sharply limit Interstitial delivery of macromolecular and nanoparticle (NP) based therapeutic agents. Collagenase-linked superparamagnetic NPs overcame these barriers and moved through in vitro extracellular matrix (ECM) at 90 μm h -1, a rate similar to invasive cells, under the influence of a magnetic field. NP migration in ECM diminished linearly over 5 days. The collagenase-NP construct overcame two of the most significant barriers to nano- and mlcroscale therapeutics deployment: proteolytic enzyme stability was maintained during a clinically useful time frame by immobilization on the NP surface and degradation of interstitial barriers to tissue biodistribution was enabled by the conjugated microbial protease.",

author = "Kuhn, {Sam J.} and Finch, {Stephanie K.} and Hallahan, {Dennis E.} and Giorgio, {Todd D.}",

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AU - Kuhn, Sam J.

AU - Finch, Stephanie K.

AU - Hallahan, Dennis E.

AU - Giorgio, Todd D.

PY - 2006/2

Y1 - 2006/2

N2 - Steric barriers such as collagen I sharply limit Interstitial delivery of macromolecular and nanoparticle (NP) based therapeutic agents. Collagenase-linked superparamagnetic NPs overcame these barriers and moved through in vitro extracellular matrix (ECM) at 90 μm h -1, a rate similar to invasive cells, under the influence of a magnetic field. NP migration in ECM diminished linearly over 5 days. The collagenase-NP construct overcame two of the most significant barriers to nano- and mlcroscale therapeutics deployment: proteolytic enzyme stability was maintained during a clinically useful time frame by immobilization on the NP surface and degradation of interstitial barriers to tissue biodistribution was enabled by the conjugated microbial protease.

AB - Steric barriers such as collagen I sharply limit Interstitial delivery of macromolecular and nanoparticle (NP) based therapeutic agents. Collagenase-linked superparamagnetic NPs overcame these barriers and moved through in vitro extracellular matrix (ECM) at 90 μm h -1, a rate similar to invasive cells, under the influence of a magnetic field. NP migration in ECM diminished linearly over 5 days. The collagenase-NP construct overcame two of the most significant barriers to nano- and mlcroscale therapeutics deployment: proteolytic enzyme stability was maintained during a clinically useful time frame by immobilization on the NP surface and degradation of interstitial barriers to tissue biodistribution was enabled by the conjugated microbial protease.

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SN - 1530-6984

VL - 6

SP - 306

EP - 312

JO - Nano Letters

JF - Nano Letters

IS - 2

ER -

Proteolytic surface functionalization enhances in vitro magnetic nanoparticle mobility through extracellular matrix

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