Abstract
Steric barriers such as collagen I sharply limit Interstitial delivery of macromolecular and nanoparticle (NP) based therapeutic agents. Collagenase-linked superparamagnetic NPs overcame these barriers and moved through in vitro extracellular matrix (ECM) at 90 μm h -1, a rate similar to invasive cells, under the influence of a magnetic field. NP migration in ECM diminished linearly over 5 days. The collagenase-NP construct overcame two of the most significant barriers to nano- and mlcroscale therapeutics deployment: proteolytic enzyme stability was maintained during a clinically useful time frame by immobilization on the NP surface and degradation of interstitial barriers to tissue biodistribution was enabled by the conjugated microbial protease.
Original language | English |
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Pages (from-to) | 306-312 |
Number of pages | 7 |
Journal | Nano Letters |
Volume | 6 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2006 |