Proteolytic surface functionalization enhances in vitro magnetic nanoparticle mobility through extracellular matrix

Sam J. Kuhn, Stephanie K. Finch, Dennis E. Hallahan, Todd D. Giorgio

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

Steric barriers such as collagen I sharply limit Interstitial delivery of macromolecular and nanoparticle (NP) based therapeutic agents. Collagenase-linked superparamagnetic NPs overcame these barriers and moved through in vitro extracellular matrix (ECM) at 90 μm h -1, a rate similar to invasive cells, under the influence of a magnetic field. NP migration in ECM diminished linearly over 5 days. The collagenase-NP construct overcame two of the most significant barriers to nano- and mlcroscale therapeutics deployment: proteolytic enzyme stability was maintained during a clinically useful time frame by immobilization on the NP surface and degradation of interstitial barriers to tissue biodistribution was enabled by the conjugated microbial protease.

Original languageEnglish
Pages (from-to)306-312
Number of pages7
JournalNano Letters
Volume6
Issue number2
DOIs
StatePublished - Feb 1 2006

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