Proteolytic activation transforms heparin cofactor II into a host defense molecule

Martina Kalle, Praveen Papareddy, Gopinath Kasetty, Douglas M. Tollefsen, Martin Malmsten, Matthias Mor̈gelin, Artur Schmidtchen

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

The abundant serine proteinase inhibitor heparin cofactor II (HCII) has been proposed to inhibit extravascular thrombin. However, the exact physiological role of this plasma protein remains enigmatic. In this study, we demonstrate a previously unknown role for HCII in host defense. Proteolytic cleavage of the molecule induced a conformational change, thereby inducing endotoxin-binding and antimicrobial properties. Analyses employing representative peptide epitopes mapped these effects to helices A and D. Mice deficient in HCII showed increased susceptibility to invasive infection by Pseudomonas aeruginosa, along with a significantly increased cytokine response. Correspondingly, decreased levels of HCII were observed in wild-type animals challenged with bacteria or endotoxin. In humans, proteolytically cleaved HCII forms were detected during wounding and in association with bacteria. Thus, the protease-induced uncovering of cryptic epitopes in HCII, which transforms the molecule into a host defense factor, represents a previously unknown regulatory mechanism in HCII biology and innate immunity.

Original languageEnglish
Pages (from-to)6303-6310
Number of pages8
JournalJournal of Immunology
Volume190
Issue number12
DOIs
StatePublished - Jun 15 2013

Fingerprint

Dive into the research topics of 'Proteolytic activation transforms heparin cofactor II into a host defense molecule'. Together they form a unique fingerprint.

Cite this