Proteins in Tumor-Derived Plasma Extracellular Vesicles Indicate Tumor Origin

Meltem Barlin; Petra Erdmann-Gilmore; Jacqueline L. Mudd; Qiang Zhang; Robert W. Seymour; Zhanfang Guo; Julia R. Miessner; S. Peter Goedegebuure; Ye Bi; Omar A. Osorio; Jennifer Alexander-Brett; Shunqiang Li; Cynthia X. Ma; Ryan C. Fields; R. Reid Townsend; Jason M. Held

doi:10.1016/j.mcpro.2022.100476

Proteins in Tumor-Derived Plasma Extracellular Vesicles Indicate Tumor Origin

Meltem Barlin, Petra Erdmann-Gilmore, Jacqueline L. Mudd, Qiang Zhang, Robert W. Seymour, Zhanfang Guo, Julia R. Miessner, S. Peter Goedegebuure, Ye Bi, Omar A. Osorio, Jennifer Alexander-Brett, Shunqiang Li, Cynthia X. Ma, Ryan C. Fields, R. Reid Townsend, Jason M. Held

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Cancer-derived extracellular vesicles (EVs) promote tumorigenesis, premetastatic niche formation, and metastasis via their protein cargo. However, the proteins packaged by patient tumors into EVs cannot be determined in vivo because of the presence of EVs derived from other tissues. We therefore developed a cross-species proteomic method to quantify the human tumor-derived proteome of plasma EVs produced by patient-derived xenografts of four cancer types. Proteomic profiling revealed individualized packaging of novel protein cargo, and machine learning accurately classified the type of the underlying tumor.

Original language	English
Article number	100476
Journal	Molecular and Cellular Proteomics
Volume	22
Issue number	1
DOIs	https://doi.org/10.1016/j.mcpro.2022.100476
State	Published - Jan 2023

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Barlin, M., Erdmann-Gilmore, P., Mudd, J. L., Zhang, Q., Seymour, R. W., Guo, Z., Miessner, J. R., Goedegebuure, S. P., Bi, Y., Osorio, O. A., Alexander-Brett, J., Li, S., Ma, C. X., Fields, R. C., Townsend, R. R., & Held, J. M. (2023). Proteins in Tumor-Derived Plasma Extracellular Vesicles Indicate Tumor Origin. Molecular and Cellular Proteomics, 22(1), Article 100476. https://doi.org/10.1016/j.mcpro.2022.100476

@article{9a79eef3dfb14029aa86eeb0d0b00194,

title = "Proteins in Tumor-Derived Plasma Extracellular Vesicles Indicate Tumor Origin",

abstract = "Cancer-derived extracellular vesicles (EVs) promote tumorigenesis, premetastatic niche formation, and metastasis via their protein cargo. However, the proteins packaged by patient tumors into EVs cannot be determined in vivo because of the presence of EVs derived from other tissues. We therefore developed a cross-species proteomic method to quantify the human tumor-derived proteome of plasma EVs produced by patient-derived xenografts of four cancer types. Proteomic profiling revealed individualized packaging of novel protein cargo, and machine learning accurately classified the type of the underlying tumor.",

author = "Meltem Barlin and Petra Erdmann-Gilmore and Mudd, {Jacqueline L.} and Qiang Zhang and Seymour, {Robert W.} and Zhanfang Guo and Miessner, {Julia R.} and Goedegebuure, {S. Peter} and Ye Bi and Osorio, {Omar A.} and Jennifer Alexander-Brett and Shunqiang Li and Ma, {Cynthia X.} and Fields, {Ryan C.} and Townsend, {R. Reid} and Held, {Jason M.}",

note = "Publisher Copyright: {\textcopyright} 2022 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).",

year = "2023",

month = jan,

doi = "10.1016/j.mcpro.2022.100476",

language = "English",

volume = "22",

journal = "Molecular and Cellular Proteomics",

issn = "1535-9476",

number = "1",

}

Barlin, M, Erdmann-Gilmore, P, Mudd, JL, Zhang, Q, Seymour, RW, Guo, Z, Miessner, JR, Goedegebuure, SP, Bi, Y, Osorio, OA, Alexander-Brett, J , Li, S , Ma, CX , Fields, RC , Townsend, RR & Held, JM 2023, 'Proteins in Tumor-Derived Plasma Extracellular Vesicles Indicate Tumor Origin', Molecular and Cellular Proteomics, vol. 22, no. 1, 100476. https://doi.org/10.1016/j.mcpro.2022.100476

TY - JOUR

T1 - Proteins in Tumor-Derived Plasma Extracellular Vesicles Indicate Tumor Origin

AU - Barlin, Meltem

AU - Erdmann-Gilmore, Petra

AU - Mudd, Jacqueline L.

AU - Zhang, Qiang

AU - Seymour, Robert W.

AU - Guo, Zhanfang

AU - Miessner, Julia R.

AU - Goedegebuure, S. Peter

AU - Bi, Ye

AU - Osorio, Omar A.

AU - Alexander-Brett, Jennifer

AU - Li, Shunqiang

AU - Ma, Cynthia X.

AU - Fields, Ryan C.

AU - Townsend, R. Reid

AU - Held, Jason M.

N1 - Publisher Copyright: © 2022 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PY - 2023/1

Y1 - 2023/1

N2 - Cancer-derived extracellular vesicles (EVs) promote tumorigenesis, premetastatic niche formation, and metastasis via their protein cargo. However, the proteins packaged by patient tumors into EVs cannot be determined in vivo because of the presence of EVs derived from other tissues. We therefore developed a cross-species proteomic method to quantify the human tumor-derived proteome of plasma EVs produced by patient-derived xenografts of four cancer types. Proteomic profiling revealed individualized packaging of novel protein cargo, and machine learning accurately classified the type of the underlying tumor.

AB - Cancer-derived extracellular vesicles (EVs) promote tumorigenesis, premetastatic niche formation, and metastasis via their protein cargo. However, the proteins packaged by patient tumors into EVs cannot be determined in vivo because of the presence of EVs derived from other tissues. We therefore developed a cross-species proteomic method to quantify the human tumor-derived proteome of plasma EVs produced by patient-derived xenografts of four cancer types. Proteomic profiling revealed individualized packaging of novel protein cargo, and machine learning accurately classified the type of the underlying tumor.

UR - http://www.scopus.com/inward/record.url?scp=85145319200&partnerID=8YFLogxK

U2 - 10.1016/j.mcpro.2022.100476

DO - 10.1016/j.mcpro.2022.100476

M3 - Article

C2 - 36470535

AN - SCOPUS:85145319200

SN - 1535-9476

VL - 22

JO - Molecular and Cellular Proteomics

JF - Molecular and Cellular Proteomics

IS - 1

M1 - 100476

ER -

Proteins in Tumor-Derived Plasma Extracellular Vesicles Indicate Tumor Origin

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