Protein Z circulates in plasma in a complex with protein Z-dependent protease inhibitor

A. Tabatabai, R. Fiehler, Jr Broze

Research output: Contribution to journalArticlepeer-review

80 Scopus citations

Abstract

Protein Z (PZ) is a vitamin K-dependent plasma protein that forms a Ca++-dependent complex with factor Xa at phospholipid surfaces. This interaction between PZ and factor Xa enhances by > 1000-fold the inhibition of factor Xa by the serpin called protein Z-dependent protease inhibitor (ZPI). These experiments show that PZ also binds ZPI in a process that does not require Ca++ or phospholipids. In pooled normal plasma, which contains excess ZPI relative to PZ, all the PZ appears to be bound in a complex with ZPI. The binding of PZ to ZPI reduces the rate and extent of factor XIa inhibition produced by ZPI. During the course of these studies, it was noted that a PZ purification procedure, that included NaSCN (2.0 M) elution of PZ from an immunoaffinity column, produced aggregated, inactive forms of PZ.

Original languageEnglish
Pages (from-to)655-660
Number of pages6
JournalThrombosis and haemostasis
Volume85
Issue number4
StatePublished - Apr 24 2001

Keywords

  • Factor XIa
  • Factor Xa
  • Protein Z
  • Protein Z-dependent protease inhibitor

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