TY - GEN
T1 - Protein-protein binding detection with nanoparticle photonic crystal enhanced microscopy (NP-PCEM)
AU - Zhuo, Yue
AU - Tian, Limei
AU - Chen, Weili
AU - Yu, Hojeong
AU - Singamaneni, Srikanth
AU - Cunningham, Brian T.
N1 - Publisher Copyright:
© 2014 IEEE.
PY - 2014/11/2
Y1 - 2014/11/2
N2 - We demonstrate a novel microscopy-based biosensing approach that utilizes a photonic crystal (PC) surface to detect protein-protein binding with the functionalized nanoparticles as tags. This imaging approach utilizes the measurement of localized shifts in the resonant wavelength and resonant reflection magnitude from the PC biosensor in the presence of individual nanoparticles. Moreover, it substantially increases the sensitivity of the imaging approach through tunable localized surface plasmon resonant frequency of the nanoparticle matching with the resonance of the PC biosensor. Experimental demonstrations of photonic crystal enhanced microscopy (PCEM) imaging with single nanoparticle resolution are supported by Finite-Difference Time-Domain (FDTD) computer simulations. The ability to detect the surface adsorption of individual nanoparticles as tags offers a route to single molecule biosensing with photonic crystal biosensor in the future.
AB - We demonstrate a novel microscopy-based biosensing approach that utilizes a photonic crystal (PC) surface to detect protein-protein binding with the functionalized nanoparticles as tags. This imaging approach utilizes the measurement of localized shifts in the resonant wavelength and resonant reflection magnitude from the PC biosensor in the presence of individual nanoparticles. Moreover, it substantially increases the sensitivity of the imaging approach through tunable localized surface plasmon resonant frequency of the nanoparticle matching with the resonance of the PC biosensor. Experimental demonstrations of photonic crystal enhanced microscopy (PCEM) imaging with single nanoparticle resolution are supported by Finite-Difference Time-Domain (FDTD) computer simulations. The ability to detect the surface adsorption of individual nanoparticles as tags offers a route to single molecule biosensing with photonic crystal biosensor in the future.
UR - http://www.scopus.com/inward/record.url?scp=84929493173&partnerID=8YFLogxK
U2 - 10.1109/EMBC.2014.6944023
DO - 10.1109/EMBC.2014.6944023
M3 - Conference contribution
C2 - 25570391
AN - SCOPUS:84929493173
T3 - 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014
SP - 2069
EP - 2072
BT - 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014
Y2 - 26 August 2014 through 30 August 2014
ER -