Protein phosphorylation in human peripheral lymphocytes - Stimulation by phytohemagglutinin and N6 monobutyryl cyclic AMP

H. James Wedner, Charles W. Parker

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22 Scopus citations

Abstract

Protein phosphorylation was studied in human peripheral lymphocytes. The cells were preincubated with {A figure is presented}, exposed to phytohemagglutinin (PHA), N6 monobutyryl cAMP (MBcAMP) or 8 bromo cGMP (BcGMP), homogenized and analyzed by SDS-polyacrylamide gel electrophoresis. Both PHA and MBcAMP produced early increases in the [32P]content of multiple proteins in the 30,000-100,000 molecular weight range. After further incubation with PHA there was a shift in the phosphorylation response to smaller molecular weight proteins. BcGMP (1 mM-10 pM) had no effect on protein phosphorylation. These results suggest a role for cAMP in the early action of PHA on human peripheral lymphocytes.

Original languageEnglish
Pages (from-to)808-815
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume62
Issue number4
DOIs
StatePublished - Feb 17 1975

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