TY - JOUR
T1 - Protein phosphorylation in human peripheral lymphocytes - Stimulation by phytohemagglutinin and N6 monobutyryl cyclic AMP
AU - James Wedner, H.
AU - Parker, Charles W.
PY - 1975/2/17
Y1 - 1975/2/17
N2 - Protein phosphorylation was studied in human peripheral lymphocytes. The cells were preincubated with {A figure is presented}, exposed to phytohemagglutinin (PHA), N6 monobutyryl cAMP (MBcAMP) or 8 bromo cGMP (BcGMP), homogenized and analyzed by SDS-polyacrylamide gel electrophoresis. Both PHA and MBcAMP produced early increases in the [32P]content of multiple proteins in the 30,000-100,000 molecular weight range. After further incubation with PHA there was a shift in the phosphorylation response to smaller molecular weight proteins. BcGMP (1 mM-10 pM) had no effect on protein phosphorylation. These results suggest a role for cAMP in the early action of PHA on human peripheral lymphocytes.
AB - Protein phosphorylation was studied in human peripheral lymphocytes. The cells were preincubated with {A figure is presented}, exposed to phytohemagglutinin (PHA), N6 monobutyryl cAMP (MBcAMP) or 8 bromo cGMP (BcGMP), homogenized and analyzed by SDS-polyacrylamide gel electrophoresis. Both PHA and MBcAMP produced early increases in the [32P]content of multiple proteins in the 30,000-100,000 molecular weight range. After further incubation with PHA there was a shift in the phosphorylation response to smaller molecular weight proteins. BcGMP (1 mM-10 pM) had no effect on protein phosphorylation. These results suggest a role for cAMP in the early action of PHA on human peripheral lymphocytes.
UR - http://www.scopus.com/inward/record.url?scp=0016637495&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(75)90394-0
DO - 10.1016/0006-291X(75)90394-0
M3 - Article
C2 - 164193
AN - SCOPUS:0016637495
SN - 0006-291X
VL - 62
SP - 808
EP - 815
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -