TY - JOUR
T1 - Protein kinase C modulates phospholipase C and increases arachidonic acid release in bradykinin stimulated MDCK cells
AU - Portilla, Didier
AU - Mordhorst, Mary
AU - Bertrand, William
AU - Morrison, Aubrey R.
N1 - Funding Information:
This work is supported by NIH Public Health Awards POI DK09976 and POI DK3811.
PY - 1988/5/31
Y1 - 1988/5/31
N2 - The tumor promoter phorbol ester (PMA) has been shown to stimulate protein kinase C (PKC) in MDCK cells. At the concentrations that produce stimulation of PKC, PMA (100μM) inhibits BK-induced I1,4,5P3 (IP3) formation and calcium transients in these cells. 1-5-isoquinolinyl-2-methyl-piperazine (H7) a known inhibitor of PKC in MDCK cells reverses the effect of PMA on BK-stimulated IP3 formation and Ca+2 transients in these cells. PMA also stimulates arachidonate release which can be inhibited by preincubation with H7. A dual mechanism of regulation by PKC at the level of phospholipase C (down regulation) and phospholipase A2 (stimulation) is suggested in these cells.
AB - The tumor promoter phorbol ester (PMA) has been shown to stimulate protein kinase C (PKC) in MDCK cells. At the concentrations that produce stimulation of PKC, PMA (100μM) inhibits BK-induced I1,4,5P3 (IP3) formation and calcium transients in these cells. 1-5-isoquinolinyl-2-methyl-piperazine (H7) a known inhibitor of PKC in MDCK cells reverses the effect of PMA on BK-stimulated IP3 formation and Ca+2 transients in these cells. PMA also stimulates arachidonate release which can be inhibited by preincubation with H7. A dual mechanism of regulation by PKC at the level of phospholipase C (down regulation) and phospholipase A2 (stimulation) is suggested in these cells.
UR - http://www.scopus.com/inward/record.url?scp=0023908502&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(88)81246-4
DO - 10.1016/S0006-291X(88)81246-4
M3 - Article
C2 - 3132168
AN - SCOPUS:0023908502
SN - 0006-291X
VL - 153
SP - 454
EP - 462
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -