Protein induced fluorescence enhancement (PIFE) for probing protein-nucleic acid interactions

Helen Hwang, Sua Myong

Research output: Contribution to journalReview articlepeer-review

164 Scopus citations

Abstract

Single molecule studies of protein-nucleic acid interactions shed light on molecular mechanisms and kinetics involved in protein binding, translocation, and unwinding of DNA and RNA substrates. In this review, we provide an overview of a single molecule fluorescence method, termed "protein induced fluorescence enhancement" (PIFE). Unlike FRET where two dyes are required, PIFE employs a single dye attached to DNA or RNA to which an unlabeled protein is applied. We discuss both ensemble and single molecule studies in which PIFE was utilized.

Original languageEnglish
Pages (from-to)1221-1229
Number of pages9
JournalChemical Society Reviews
Volume43
Issue number4
DOIs
StatePublished - Feb 21 2014

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