Protein array-based profiling of CSF identifies RBPJ as an autoantigen in multiple sclerosis

Luis Querol, Pamela L. Clark, Mary A. Bailey, Chris Cotsapas, Anne H. Cross, David A. Hafler, Steven H. Kleinstein, Jae Yun Lee, Gur Yaari, Simon N. Willis, Kevin C. O'Connor

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Objective: To profile the reactivity of CSF-derived immunoglobulin from patients with multiple sclerosis (MS) against a large panel of antigens, to identify disease-specific reactivities. Methods: CSF from subjects with MS with elevated immunoglobulin G and CSF from control subjects presenting with other inflammatory neurologic disease were screened against a protein array consisting of 9,393 proteins. Reactivity to a candidate protein identified using these arrays was confirmed with ELISA and immunocytochemistry. Results: Autoantibodies against one protein on the array, recombination signal binding protein for immunoglobulin kappa J region (RBPJ), discriminated between patients with MS and controls (p 5 0.0052). Using a large validation cohort, we found a higher prevalence of autoantibodies against RBPJ in the CSF of patients with MS (12.5%) compared with the CSF of patients with other neurologic diseases (1.6%; p 5 0.02) by ELISA. This difference in reactivity was restricted to the CSF as serum reactivity against RBPJ did not differ between patients and controls. The presence of CSF autoantibodies against RBPJ was further confirmed by immunocytochemistry. Conclusions: These data indicate that RBPJ, a ubiquitous protein of the Notch signaling pathway that plays an important role in Epstein-Barr virus infection, is a novel MS autoantigen candidate that is recognized by CSF-derived immunoglobulin G in a subset of patients with MS.

Original languageEnglish
Pages (from-to)956-963
Number of pages8
JournalNeurology
Volume81
Issue number11
DOIs
StatePublished - Sep 10 2013

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