Protective T cell immunity in mice following protein-TLR7/8 agonist-conjugate immunization requires aggregation, type I IFN, and multiple DC subsets

Kathrin Kastenmüller, Ulrike Wille-Reece, Ross W.B. Lindsay, Lauren R. Trager, Patricia A. Darrah, Barbara J. Flynn, Maria R. Becker, Mark C. Udey, Björn E. Clausen, Botond Z. Igyarto, Daniel H. Kaplan, Wolfgang Kastenmüller, Ronald N. Germain, Robert A. Seder

Research output: Contribution to journalArticle

115 Scopus citations

Abstract

The success of a non-live vaccine requires improved formulation and adjuvant selection to generate robust T cell immunity following immunization. Here, using protein linked to a TLR7/8 agonist (conjugate vaccine), we investigated the functional properties of vaccine formulation, the cytokines, and the DC subsets required to induce protective multifunctional T cell immunity in vivo. The conjugate vaccine required aggregation of the protein to elicit potent Th1 CD4+ and CD8+ T cell responses. Remarkably, the conjugate vaccine, through aggregation of the protein and activation of TLR7 in vivo, led to an influx of migratory DCs to the LN and increased antigen uptake by several resident and migratory DC subsets, with the latter effect strongly influenced by vaccine-induced type I IFN. Ex vivo migratory CD8 -DEC205+CD103-CD326- langerin-negative dermal DCs were as potent in cross-presenting antigen to naive CD8+ T cells as CD11c+CD8+ DCs. Moreover, these cells also influenced Th1 CD4+ T cell priming. In summary, we propose a model in which broad-based T cell-mediated responses upon vaccination can be maximized by codelivery of aggregated protein and TLR7/8 agonist, which together promote optimal antigen acquisition and presentation by multiple DC subsets in the context of critical proinflammatory cytokines.

Original languageEnglish
Pages (from-to)1782-1796
Number of pages15
JournalJournal of Clinical Investigation
Volume121
Issue number5
DOIs
StatePublished - May 2 2011
Externally publishedYes

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    Kastenmüller, K., Wille-Reece, U., Lindsay, R. W. B., Trager, L. R., Darrah, P. A., Flynn, B. J., Becker, M. R., Udey, M. C., Clausen, B. E., Igyarto, B. Z., Kaplan, D. H., Kastenmüller, W., Germain, R. N., & Seder, R. A. (2011). Protective T cell immunity in mice following protein-TLR7/8 agonist-conjugate immunization requires aggregation, type I IFN, and multiple DC subsets. Journal of Clinical Investigation, 121(5), 1782-1796. https://doi.org/10.1172/JCI45416