Prostaglandin and thromboxane synthesis by microsomes of inflamed rabbit ciliary body-iris

M. A. Kass, N. J. Holmberg, M. E. Smith

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Microsomes of albino rabbit ciliary body-iris were prepared 6 hr, 24 hr, 3 days, 7 days, and 28 days after intravitreal injection of 10 μg of Shigella endotoxin. The microsomal preparations were incubated for 15 min with [I-14C]arachidonic acid. Prostaglandin and thromboxane products (cyclo-oxygenase products) were identified by thin-layer chromatography and quantified by scintillation counting. Synthesis of prostaglandin F(2α) (PGF(2α)), PGE2, PGD2, 6-keto-PGF(1α) (a stable metabolite of PGI2) and thromboxane B2 (TXB2) (a stable metabolite of TXA2) was increased 24 hr, 3 days, and 7 days after endotoxin injection. The greatest increase was in TXB2 synthesis. Cyclo-oxygenase product synthesis returned to normal levels by 28 days. Ciliary body-iris microsomes prepared 15 min after paracentesis synthesized increased amounts of all cyclo-oxygenase products assayed, most notably TXB2 and PGE2. Ciliary body-iris microsomes from albino rabbits treated with topical 1% nitrogen mustard or pigmented rabbits treated with subcutaneous alpha-melanocyte-stimulating hormone (20 μg/kg) synthesized normal amounts of cyclo-oxygenase products.

Original languageEnglish
Pages (from-to)442-449
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume20
Issue number4
StatePublished - Jan 1 1981

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