TY - JOUR
T1 - Prostaglandin and thromboxane synthesis by microsomes of inflamed rabbit ciliary body-iris
AU - Kass, M. A.
AU - Holmberg, N. J.
AU - Smith, M. E.
PY - 1981
Y1 - 1981
N2 - Microsomes of albino rabbit ciliary body-iris were prepared 6 hr, 24 hr, 3 days, 7 days, and 28 days after intravitreal injection of 10 μg of Shigella endotoxin. The microsomal preparations were incubated for 15 min with [I-14C]arachidonic acid. Prostaglandin and thromboxane products (cyclo-oxygenase products) were identified by thin-layer chromatography and quantified by scintillation counting. Synthesis of prostaglandin F(2α) (PGF(2α)), PGE2, PGD2, 6-keto-PGF(1α) (a stable metabolite of PGI2) and thromboxane B2 (TXB2) (a stable metabolite of TXA2) was increased 24 hr, 3 days, and 7 days after endotoxin injection. The greatest increase was in TXB2 synthesis. Cyclo-oxygenase product synthesis returned to normal levels by 28 days. Ciliary body-iris microsomes prepared 15 min after paracentesis synthesized increased amounts of all cyclo-oxygenase products assayed, most notably TXB2 and PGE2. Ciliary body-iris microsomes from albino rabbits treated with topical 1% nitrogen mustard or pigmented rabbits treated with subcutaneous alpha-melanocyte-stimulating hormone (20 μg/kg) synthesized normal amounts of cyclo-oxygenase products.
AB - Microsomes of albino rabbit ciliary body-iris were prepared 6 hr, 24 hr, 3 days, 7 days, and 28 days after intravitreal injection of 10 μg of Shigella endotoxin. The microsomal preparations were incubated for 15 min with [I-14C]arachidonic acid. Prostaglandin and thromboxane products (cyclo-oxygenase products) were identified by thin-layer chromatography and quantified by scintillation counting. Synthesis of prostaglandin F(2α) (PGF(2α)), PGE2, PGD2, 6-keto-PGF(1α) (a stable metabolite of PGI2) and thromboxane B2 (TXB2) (a stable metabolite of TXA2) was increased 24 hr, 3 days, and 7 days after endotoxin injection. The greatest increase was in TXB2 synthesis. Cyclo-oxygenase product synthesis returned to normal levels by 28 days. Ciliary body-iris microsomes prepared 15 min after paracentesis synthesized increased amounts of all cyclo-oxygenase products assayed, most notably TXB2 and PGE2. Ciliary body-iris microsomes from albino rabbits treated with topical 1% nitrogen mustard or pigmented rabbits treated with subcutaneous alpha-melanocyte-stimulating hormone (20 μg/kg) synthesized normal amounts of cyclo-oxygenase products.
UR - http://www.scopus.com/inward/record.url?scp=0019509526&partnerID=8YFLogxK
M3 - Article
C2 - 7216665
AN - SCOPUS:0019509526
SN - 0146-0404
VL - 20
SP - 442
EP - 449
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
IS - 4
ER -