TY - JOUR
T1 - Prospective use of molecular typing of Mycobacterium tuberculosis by use of restriction fragment-length polymorphism in a public tuberculosis-control program
AU - McConkey, Samuel J.
AU - Williams, Michael
AU - Weiss, Don
AU - Adams, Hilda
AU - Cave, M. Donald
AU - Yang, Zhenhua
AU - Lindner, Tom
AU - Bailey, Thomas C.
N1 - Funding Information:
Financial support: Centers for Disease Control and Prevention; Veterans Administration (interagency agreements 98FED10318-09 and V598(90)S9304); and National Heart, Lung, and Blood Institute (TB Academic Award HL03583).
PY - 2002/3
Y1 - 2002/3
N2 - We performed a prospective, community-based evaluation of molecular typing of Mycobacterium tuberculosis isolates as a method for tuberculosis (TB) control. We performed restriction fragment - length polymorphism (RFLP) analysis of the insertion sequences IS6110 and pTBN12 for isolates recovered from 61 of 62 patients with culture-positive TB in St. Louis during 12 months. Twenty-four (39%) of the 61 patients were infected with an isolate with an RFLP pattern that was shared with ≥1 other isolate, and 11 (46%) also had epidemiologic links with patients in their cluster of cases. One case each of laboratory cross-contamination and occupational transmission were discovered. The patients in dusters were more likely to be younger, black, United States - born, to have substance abuse problems, and to live in poorer areas. A predictive algorithm for molecular identification of clusters had a sensitivity and a specificity of 75%. This study allowed the TB-control program in St. Louis to be redirected toward the affected subpopulations.
AB - We performed a prospective, community-based evaluation of molecular typing of Mycobacterium tuberculosis isolates as a method for tuberculosis (TB) control. We performed restriction fragment - length polymorphism (RFLP) analysis of the insertion sequences IS6110 and pTBN12 for isolates recovered from 61 of 62 patients with culture-positive TB in St. Louis during 12 months. Twenty-four (39%) of the 61 patients were infected with an isolate with an RFLP pattern that was shared with ≥1 other isolate, and 11 (46%) also had epidemiologic links with patients in their cluster of cases. One case each of laboratory cross-contamination and occupational transmission were discovered. The patients in dusters were more likely to be younger, black, United States - born, to have substance abuse problems, and to live in poorer areas. A predictive algorithm for molecular identification of clusters had a sensitivity and a specificity of 75%. This study allowed the TB-control program in St. Louis to be redirected toward the affected subpopulations.
UR - http://www.scopus.com/inward/record.url?scp=0036498809&partnerID=8YFLogxK
U2 - 10.1086/338785
DO - 10.1086/338785
M3 - Article
C2 - 11807682
AN - SCOPUS:0036498809
VL - 34
SP - 612
EP - 619
JO - Clinical Infectious Diseases
JF - Clinical Infectious Diseases
SN - 1058-4838
IS - 5
ER -