Proprioceptive afferents survive in the masseter muscle of trkC knockout mice

S. Matsuo, H. Ichikawa, I. Silos-Santiago, J. J.A. Arends, T. A. Henderson, K. Kiyomiya, M. Kurebe, M. F. Jacquin

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Peripheral innervation patterns of proprioceptive afferents from dorsal root ganglia and the mesencephalic trigeminal nucleus were assessed in trkC- deficient mice using immunohistochemistry for protein gene product 9.5 and parvalbumin. In trkC knockout mice, spinal proprioceptive afferents were completely absent in the limb skeletal muscles, M. biceps femoris and M. gastrocnemius, as previously reported. In these same animals, however, proprioceptive afferents from mesencephalic trigeminal nucleus innervated masseter muscles and formed primary endings of muscle spindles. Three wild- type mice averaged 35.7 spindle profiles (range: 31-41), six heterozygotes averaged 32.3 spindles (range: 27-41), and four homozygotes averaged 32.8 spindles (range: 26-42). Parvalbumin and Nissl staining of the brain stem showed approximately 50% surviving mesencephalic trigeminal sensory neurons in trkC-deficient mice. TrkC -/- mice (n = 5) had 309.4 ± 15.9 mesencephalic trigeminal sensory cells versus 616.5 ± 26.3 the sensory cells in trkC +/+ mice (n = 4). These data indicate that while mesencephalic trigeminal sensory neurons are significantly reduced in number by trkC deletion, they are not completely absent. Furthermore, unlike their spinal counterparts, trigeminal proprioceptive afferents survive and give rise to stretch receptor complexes in masseter muscles of trkC knockout mice. This indicates that spinal and mesencephalic trigeminal proprioceptive afferents have different neurotrophin-supporting system during survival and differentiation. It is likely that one or more other neurotrophin receptors expressed in mesencephalic trigeminal proprioceptive neurons of trkC knockout mice compensate for the lack of normal neurotrophin-3 signaling through trkC.

Original languageEnglish
Pages (from-to)209-216
Number of pages8
JournalNeuroscience
Volume95
Issue number1
DOIs
StatePublished - Nov 1 1999

Keywords

  • Masseter muscle
  • Mesencephalic trigeminal nucleus
  • Muscle spindle
  • Mutant mice
  • Proprioceptive afferent
  • trkC

Fingerprint Dive into the research topics of 'Proprioceptive afferents survive in the masseter muscle of trkC knockout mice'. Together they form a unique fingerprint.

  • Cite this

    Matsuo, S., Ichikawa, H., Silos-Santiago, I., Arends, J. J. A., Henderson, T. A., Kiyomiya, K., Kurebe, M., & Jacquin, M. F. (1999). Proprioceptive afferents survive in the masseter muscle of trkC knockout mice. Neuroscience, 95(1), 209-216. https://doi.org/10.1016/S0306-4522(99)00424-8