Properties of mutants of Synechocystis sp. strain PCC 6803 lacking inorganic carbon sequestration systems

  • Min Xu
  • , Gábor Bernát
  • , Abhay Singh
  • , Hualing Mi
  • , Matthias Rögner
  • , Himadri B. Pakrasi
  • , Teruo Ogawa

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

A mutant (Δ5) of Synechocystis sp. strain PCC 6803 constructed by inactivating five inorganic carbon sequestration systems did not take up CO 2 or HCO3- and was unable to grow in air with or without glucose. The Δ4 mutant in which BicA is the only active inorganic carbon sequestration system showed low activity of HCO 3- uptake and grew under these conditions but more slowly than the wild-type strain. The Δ5 mutant required 1.7% CO2 to attain half the maximal growth rate. Electron transport activity of the mutants was strongly inhibited under high light intensities, with the Δ5 mutant more susceptible to high light than the Δ4 mutant. The results implicated the significance of carbon sequestration in dissipating excess light energy.

Original languageEnglish
Pages (from-to)1672-1677
Number of pages6
JournalPlant and Cell Physiology
Volume49
Issue number11
DOIs
StatePublished - Nov 2008

Keywords

  • CO -concentrating mechanism (CCM)
  • CO and HCO uptake
  • Cyanobacteria
  • Electron transport
  • Inorganic carbon sequestration

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