TY - JOUR
T1 - Promyelinating Schwann cells express Tst-1/SCIP/Oct-6
AU - Arroyo, Edgardo J.
AU - Bermingham, John R.
AU - Rosenfeld, Michael G.
AU - Scherer, Steven S.
PY - 1998/10/1
Y1 - 1998/10/1
N2 - Tst-1/SCIP/Oct-6, a POU domain transcription factor, is transiently expressed by developing Schwann cells and is required for their normal development into a myelinating phenotype. In tst-1/scip/oct-6-null sciatic nerves, Schwann cells are transiently arrested at the 'promyelinating' stage, when they have a one-to-one relationship with an axon but before they have elaborated a myelin sheath. To determine when Schwann cells express Tst- 1/SCIP/Oct-6, we examined β-galactosidase (β-gal) expression in heterozygous tst-1/scip/oct-6 mice, in which one copy of the tst-1/scip/oct- 6 gene has been replaced with the LacZ gene. β-Gal expression from the LacZ gene seems to parallel Tst-1/SCIP/Oct-6 expression from the endogenous tst- 1/scip/oct-6 gene in developing and regenerating sciatic nerves. Furthermore, electron microscopic examination of 5-bromo-4-chloro-3-indolyl-β-D- galactopyranoside- (X-gal) and halogenated indolyl-β-D-galactoside- (Bluo- gal) stained nerves showed that promyelinating Schwann cells express the highest levels of β-gal, both in developing and in regenerating nerves. Thus, the expression of β-gal, a surrogate marker of Tst-1/SCIP/Oct-6, peaks at the same stage of Schwann cell development at which development is arrested in tst-1/scip/oct-6-null mice, indicating that Tst-1/SCIP/Oct-6 has a critical role in promyelinating Schwann cells.
AB - Tst-1/SCIP/Oct-6, a POU domain transcription factor, is transiently expressed by developing Schwann cells and is required for their normal development into a myelinating phenotype. In tst-1/scip/oct-6-null sciatic nerves, Schwann cells are transiently arrested at the 'promyelinating' stage, when they have a one-to-one relationship with an axon but before they have elaborated a myelin sheath. To determine when Schwann cells express Tst- 1/SCIP/Oct-6, we examined β-galactosidase (β-gal) expression in heterozygous tst-1/scip/oct-6 mice, in which one copy of the tst-1/scip/oct- 6 gene has been replaced with the LacZ gene. β-Gal expression from the LacZ gene seems to parallel Tst-1/SCIP/Oct-6 expression from the endogenous tst- 1/scip/oct-6 gene in developing and regenerating sciatic nerves. Furthermore, electron microscopic examination of 5-bromo-4-chloro-3-indolyl-β-D- galactopyranoside- (X-gal) and halogenated indolyl-β-D-galactoside- (Bluo- gal) stained nerves showed that promyelinating Schwann cells express the highest levels of β-gal, both in developing and in regenerating nerves. Thus, the expression of β-gal, a surrogate marker of Tst-1/SCIP/Oct-6, peaks at the same stage of Schwann cell development at which development is arrested in tst-1/scip/oct-6-null mice, indicating that Tst-1/SCIP/Oct-6 has a critical role in promyelinating Schwann cells.
KW - Axon-Schwann cell interactions
KW - Myelin
KW - Neuropathy
KW - POU
KW - Peripheral nerve
KW - Transcription factors
KW - cAMP
UR - http://www.scopus.com/inward/record.url?scp=0032188799&partnerID=8YFLogxK
U2 - 10.1523/jneurosci.18-19-07891.1998
DO - 10.1523/jneurosci.18-19-07891.1998
M3 - Article
C2 - 9742157
AN - SCOPUS:0032188799
SN - 0270-6474
VL - 18
SP - 7891
EP - 7902
JO - Journal of Neuroscience
JF - Journal of Neuroscience
IS - 19
ER -