Promotion of incisional wound repair by human mesenchymal stem cell transplantation

Alexander Stoff; Angel A. Rivera; N. Sanjib Banerjee; Steven T. Moore; T. Michael Numnum; Antonio Espinosa-de-los-Monteros; Dirk F. Richter; Gene P. Siegal; Louise T. Chow; Dale Feldman; Luis O. Vasconez; J. Michael Mathis; Mariam A. Stoff-Khalili; David T. Curiel

doi:10.1111/j.1600-0625.2008.00792.x

Promotion of incisional wound repair by human mesenchymal stem cell transplantation

Alexander Stoff, Angel A. Rivera, N. Sanjib Banerjee, Steven T. Moore, T. Michael Numnum, Antonio Espinosa-de-los-Monteros, Dirk F. Richter, Gene P. Siegal, Louise T. Chow, Dale Feldman, Luis O. Vasconez, J. Michael Mathis, Mariam A. Stoff-Khalili, David T. Curiel

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111 Scopus citations

Abstract

The purpose of this study was to determine the effect of transplanted human mesenchymal stem cells (hMSCs) on wound healing. In this model, full-thickness cutaneous wounds were created by incision in the skin of adult New Zealand white rabbits and treated by transplanted hMSCs into the wounds. Wound healing was evaluated by histological analysis and tensiometry over time. A total of 15 New Zealand white rabbits with 10 wounds per animal were examined in this study. Animals were treated with hMSCs and euthanised at 3, 7, 14, 21 and 80 days after manipulation. The hMSCs were labelled with a fluorescent dye (CM-DiI), suspended in phosphate-buffered saline and used to treat full-thickness incisional wounds in rabbit skin. Tensiometry and histology were used to characterise the wound-healing rate of the incisional wounds. These results showed that transplanted hMSCs significantly inhibited scar formation and increased the tensile strength of the wounds. Importantly, MSCs from genetically unrelated donors did not appear to induce an immunologic response. In conclusion, human mesenchymal stem cell therapy is a viable approach to significantly affect the course of normal cutaneous wound healing and significantly increase the tensile strength.

Original language	English
Pages (from-to)	362-369
Number of pages	8
Journal	Experimental Dermatology
Volume	18
Issue number	4
DOIs	https://doi.org/10.1111/j.1600-0625.2008.00792.x
State	Published - 2009

Keywords

Incisional wounds
Mesenchymal stem cells
Scar formation
Tensile strength
Wound healing

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10.1111/j.1600-0625.2008.00792.x

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Stoff, A., Rivera, A. A., Banerjee, N. S., Moore, S. T., Numnum, T. M., Espinosa-de-los-Monteros, A., Richter, D. F., Siegal, G. P., Chow, L. T., Feldman, D., Vasconez, L. O., Mathis, J. M., Stoff-Khalili, M. A., & Curiel, D. T. (2009). Promotion of incisional wound repair by human mesenchymal stem cell transplantation. Experimental Dermatology, 18(4), 362-369. https://doi.org/10.1111/j.1600-0625.2008.00792.x

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title = "Promotion of incisional wound repair by human mesenchymal stem cell transplantation",

abstract = "The purpose of this study was to determine the effect of transplanted human mesenchymal stem cells (hMSCs) on wound healing. In this model, full-thickness cutaneous wounds were created by incision in the skin of adult New Zealand white rabbits and treated by transplanted hMSCs into the wounds. Wound healing was evaluated by histological analysis and tensiometry over time. A total of 15 New Zealand white rabbits with 10 wounds per animal were examined in this study. Animals were treated with hMSCs and euthanised at 3, 7, 14, 21 and 80 days after manipulation. The hMSCs were labelled with a fluorescent dye (CM-DiI), suspended in phosphate-buffered saline and used to treat full-thickness incisional wounds in rabbit skin. Tensiometry and histology were used to characterise the wound-healing rate of the incisional wounds. These results showed that transplanted hMSCs significantly inhibited scar formation and increased the tensile strength of the wounds. Importantly, MSCs from genetically unrelated donors did not appear to induce an immunologic response. In conclusion, human mesenchymal stem cell therapy is a viable approach to significantly affect the course of normal cutaneous wound healing and significantly increase the tensile strength.",

keywords = "Incisional wounds, Mesenchymal stem cells, Scar formation, Tensile strength, Wound healing",

author = "Alexander Stoff and Rivera, {Angel A.} and Banerjee, {N. Sanjib} and Moore, {Steven T.} and Numnum, {T. Michael} and Antonio Espinosa-de-los-Monteros and Richter, {Dirk F.} and Siegal, {Gene P.} and Chow, {Louise T.} and Dale Feldman and Vasconez, {Luis O.} and Mathis, {J. Michael} and Stoff-Khalili, {Mariam A.} and Curiel, {David T.}",

year = "2009",

doi = "10.1111/j.1600-0625.2008.00792.x",

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Stoff, A, Rivera, AA, Banerjee, NS, Moore, ST, Numnum, TM, Espinosa-de-los-Monteros, A, Richter, DF, Siegal, GP, Chow, LT, Feldman, D, Vasconez, LO, Mathis, JM, Stoff-Khalili, MA & Curiel, DT 2009, 'Promotion of incisional wound repair by human mesenchymal stem cell transplantation', Experimental Dermatology, vol. 18, no. 4, pp. 362-369. https://doi.org/10.1111/j.1600-0625.2008.00792.x

TY - JOUR

T1 - Promotion of incisional wound repair by human mesenchymal stem cell transplantation

AU - Stoff, Alexander

AU - Rivera, Angel A.

AU - Banerjee, N. Sanjib

AU - Moore, Steven T.

AU - Numnum, T. Michael

AU - Espinosa-de-los-Monteros, Antonio

AU - Richter, Dirk F.

AU - Siegal, Gene P.

AU - Chow, Louise T.

AU - Feldman, Dale

AU - Vasconez, Luis O.

AU - Mathis, J. Michael

AU - Stoff-Khalili, Mariam A.

AU - Curiel, David T.

PY - 2009

Y1 - 2009

N2 - The purpose of this study was to determine the effect of transplanted human mesenchymal stem cells (hMSCs) on wound healing. In this model, full-thickness cutaneous wounds were created by incision in the skin of adult New Zealand white rabbits and treated by transplanted hMSCs into the wounds. Wound healing was evaluated by histological analysis and tensiometry over time. A total of 15 New Zealand white rabbits with 10 wounds per animal were examined in this study. Animals were treated with hMSCs and euthanised at 3, 7, 14, 21 and 80 days after manipulation. The hMSCs were labelled with a fluorescent dye (CM-DiI), suspended in phosphate-buffered saline and used to treat full-thickness incisional wounds in rabbit skin. Tensiometry and histology were used to characterise the wound-healing rate of the incisional wounds. These results showed that transplanted hMSCs significantly inhibited scar formation and increased the tensile strength of the wounds. Importantly, MSCs from genetically unrelated donors did not appear to induce an immunologic response. In conclusion, human mesenchymal stem cell therapy is a viable approach to significantly affect the course of normal cutaneous wound healing and significantly increase the tensile strength.

AB - The purpose of this study was to determine the effect of transplanted human mesenchymal stem cells (hMSCs) on wound healing. In this model, full-thickness cutaneous wounds were created by incision in the skin of adult New Zealand white rabbits and treated by transplanted hMSCs into the wounds. Wound healing was evaluated by histological analysis and tensiometry over time. A total of 15 New Zealand white rabbits with 10 wounds per animal were examined in this study. Animals were treated with hMSCs and euthanised at 3, 7, 14, 21 and 80 days after manipulation. The hMSCs were labelled with a fluorescent dye (CM-DiI), suspended in phosphate-buffered saline and used to treat full-thickness incisional wounds in rabbit skin. Tensiometry and histology were used to characterise the wound-healing rate of the incisional wounds. These results showed that transplanted hMSCs significantly inhibited scar formation and increased the tensile strength of the wounds. Importantly, MSCs from genetically unrelated donors did not appear to induce an immunologic response. In conclusion, human mesenchymal stem cell therapy is a viable approach to significantly affect the course of normal cutaneous wound healing and significantly increase the tensile strength.

KW - Incisional wounds

KW - Mesenchymal stem cells

KW - Scar formation

KW - Tensile strength

KW - Wound healing

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JO - Experimental Dermatology

JF - Experimental Dermatology

IS - 4

ER -

Promotion of incisional wound repair by human mesenchymal stem cell transplantation

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Keywords

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