TY - CHAP
T1 - PROMOTER OPTIMIZATION AND ARTIFICIAL PROMOTERS FOR TRANSCRIPTIONAL TARGETING IN GENE THERAPY
AU - Nettelbeck, Dirk M.
AU - Curiel, David T.
AU - Müller, Rolf
N1 - Publisher Copyright:
© 2002 by John Wiley & Sons, Inc. All rights reserved.
PY - 2003/1/1
Y1 - 2003/1/1
N2 - Transcriptional targeting facilitates spatial, inducible or physiologically regulated gene therapy or virotherapy by the utilization of regulatory DNA sequences to drive expression of therapeutic or viral genes. Specific, effective, and vector-compatible promoters are key to the realization of the full potential of transcriptional targeting. Few promoters that fulfill these requirements have been described and for some applications suitable natural promoters might not exist. This chapter reviews strategies developed to overcome these limitations: optimization of natural promoters, design of artificial promoters, and adaptation of vector backbones. These endeavors aim to develop advanced vectors required for future clinical applications.
AB - Transcriptional targeting facilitates spatial, inducible or physiologically regulated gene therapy or virotherapy by the utilization of regulatory DNA sequences to drive expression of therapeutic or viral genes. Specific, effective, and vector-compatible promoters are key to the realization of the full potential of transcriptional targeting. Few promoters that fulfill these requirements have been described and for some applications suitable natural promoters might not exist. This chapter reviews strategies developed to overcome these limitations: optimization of natural promoters, design of artificial promoters, and adaptation of vector backbones. These endeavors aim to develop advanced vectors required for future clinical applications.
UR - http://www.scopus.com/inward/record.url?scp=85218300746&partnerID=8YFLogxK
U2 - 10.1002/0471234303.ch22
DO - 10.1002/0471234303.ch22
M3 - Chapter
AN - SCOPUS:85218300746
SN - 9780471434795
SP - 481
EP - 503
BT - Vector Targeting for Therapeutic Gene Delivery
PB - wiley
ER -