Prolyl hydroxylase 2: A novel regulator of β 2-adrenoceptor internalization

Biao Yan, Zhaoxia Huo, Ying Liu, Xiaoping Lin, Jun Li, Luying Peng, Hong Zhao, Zhao Nian Zhou, Xingqun Liang, Yi Liu, Weidong Zhu, Dandan Liang, Li Li, Yunfu Sun, Jianmin Cui, Yi Han Chen

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Adrenergic receptor (AR)-mediated signalling is modulated by oxygen levels. Prolyl hydroxylases (PHDs) are crucial for intracellular oxygen sensing and organism survival. However, it remains to be clarified whether or how PHDs are involved in the regulation of β 2-adrenoceptor (β 2-AR) signalling. Here we show that PHD2 can modulate the rate of β 2-AR internalization through interactions with β-arrestin 2. PHD2 hydroxylates β-arrestin 2 at the proline (Pro) 176, Pro 179 and Pro 181 sites, which retards the recruitment of β-arrestin 2 to the plasma membrane and inhibits subsequent co-internalization with β 2-AR into the cytosol. β 2-AR internalization is critical to control the temporal and spatial aspects of β 2-AR signalling. Identifying novel regulators of β 2-AR internalization will enable us to develop new strategies to manipulate receptor signalling and provide potential targets for drug development in the prevention and treatment of diseases associated with β 2-AR signalling dysregulation.

Original languageEnglish
Pages (from-to)2712-2722
Number of pages11
JournalJournal of Cellular and Molecular Medicine
Issue number12
StatePublished - Dec 2011


  • Hydroxylation
  • Internalization
  • Prolyl hydroxylase
  • β -adrenoceptor
  • β-arrestin 2


Dive into the research topics of 'Prolyl hydroxylase 2: A novel regulator of β 2-adrenoceptor internalization'. Together they form a unique fingerprint.

Cite this