TY - JOUR
T1 - Products derived from 5,8,11-eicosatrienoic acid by the 5-lipoxygenase-leukotriene pathway
AU - Jakschik, B. A.
AU - Morrison, A. R.
AU - Sprecher, H.
PY - 1983
Y1 - 1983
N2 - Analysis of products derived from 5,8,11-eicosatrienoic acid via the 5-lipoxygenase-leukotriene pathway showed that this fatty acid is readily converted to leukotriene (LT)A3. When 10,000 x g supernatant from rat basophilic leukemia cell homogenates was incubated with 30 μM fatty acid, 5,8,11-eicosatrienoic acid produced 6.2 ± 1.1 nmol of LTA3 and arachidonic acid 15.5 ± 1.9 nmol of LTA4 (n = 4). However, only insignificant amounts of LTB3 were formed (0.15 ± 0.04 nmol of LTB3 and 4.2 ± 0.4 nmol of LTB4, n = 4). These data indicate that the LTA-hydrolase requires not only the three double bonds of the triene but also the double bond at C-14 to efficiently convert LTA to LTB. These findings have significant implications for essential fatty acid deficiency.
AB - Analysis of products derived from 5,8,11-eicosatrienoic acid via the 5-lipoxygenase-leukotriene pathway showed that this fatty acid is readily converted to leukotriene (LT)A3. When 10,000 x g supernatant from rat basophilic leukemia cell homogenates was incubated with 30 μM fatty acid, 5,8,11-eicosatrienoic acid produced 6.2 ± 1.1 nmol of LTA3 and arachidonic acid 15.5 ± 1.9 nmol of LTA4 (n = 4). However, only insignificant amounts of LTB3 were formed (0.15 ± 0.04 nmol of LTB3 and 4.2 ± 0.4 nmol of LTB4, n = 4). These data indicate that the LTA-hydrolase requires not only the three double bonds of the triene but also the double bond at C-14 to efficiently convert LTA to LTB. These findings have significant implications for essential fatty acid deficiency.
UR - http://www.scopus.com/inward/record.url?scp=0021039332&partnerID=8YFLogxK
M3 - Article
C2 - 6313677
AN - SCOPUS:0021039332
SN - 0021-9258
VL - 258
SP - 12797
EP - 12800
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 21
ER -