Products derived from 5,8,11-eicosatrienoic acid by the 5-lipoxygenase-leukotriene pathway

B. A. Jakschik, A. R. Morrison, H. Sprecher

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Analysis of products derived from 5,8,11-eicosatrienoic acid via the 5-lipoxygenase-leukotriene pathway showed that this fatty acid is readily converted to leukotriene (LT)A3. When 10,000 x g supernatant from rat basophilic leukemia cell homogenates was incubated with 30 μM fatty acid, 5,8,11-eicosatrienoic acid produced 6.2 ± 1.1 nmol of LTA3 and arachidonic acid 15.5 ± 1.9 nmol of LTA4 (n = 4). However, only insignificant amounts of LTB3 were formed (0.15 ± 0.04 nmol of LTB3 and 4.2 ± 0.4 nmol of LTB4, n = 4). These data indicate that the LTA-hydrolase requires not only the three double bonds of the triene but also the double bond at C-14 to efficiently convert LTA to LTB. These findings have significant implications for essential fatty acid deficiency.

Original languageEnglish
Pages (from-to)12797-12800
Number of pages4
JournalJournal of Biological Chemistry
Volume258
Issue number21
StatePublished - 1983

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