Productive infection of CD34+-cell-derived megakaryocytes by X4 and R5 HIV-1 isolates

Frosso Voulgaropoulou, Suzanne E. Pontow, Lee Ratner

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

The human immunodeficiency virus (HIV-1) causes various hematopoietic abnormalities, with thrombocytopenia (TP) occurring in 30% of infected individuals. In the present study, we aimed to determine whether HIV-1 in the bone marrow of TP patients can infect primary megakaryocytes in vitro, which may contribute to the development of thrombocytopenia. We amplified the V3 loop of HIV-1 envelope from the bone marrow of TP and non-TP patients and constructed recombinant viruses. We demonstrate that the bone marrow of TP and non-TP patients contained R5 strains, whereas X4 strains were present only in the bone marrow of TP patients. Furthermore, HIV-1 from the bone marrow of TP and non-TP patients infected megakaryocytes to similar levels, suggesting that the V3 loop of HIV-1 may not contain the viral determinants of HIV-associated TP. Chemokine receptor analysis determined that CD34+-cell- derived megakaryocytes express CD4, CXCR4, and CCR5 and are productively infected by both X4 and R5 HIV-1 isolates. Finally, we showed that CD34+- cell-derived megakaryocytes express the chemokine receptor CCR3. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)78-85
Number of pages8
JournalVirology
Volume269
Issue number1
DOIs
StatePublished - Mar 30 2000

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