Abstract
Procedures detailing the production of monoclonal antibody supernatants, including the production of larger amounts (liters) are presented in this unit. An alternate protocol for large-scale production of hybridomas or cells (e.g., for isolation of cellular proteins) involves a similar procedure. A method for producing and obtaining ascites fluid containing the monoclonal antibody is also presented.
| Original language | English |
|---|---|
| Pages (from-to) | Unit11.10 |
| Journal | Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.] |
| Volume | Chapter 11 |
| DOIs | |
| State | Published - May 2001 |