TY - JOUR
T1 - Production of monoclonal antibodies
AU - Yokoyama, Wayne M.
AU - Christensen, Michelle
AU - Santos, Gary Dos
AU - Miller, Diane
AU - Ho, Jason
AU - Wu, Tao
AU - Dziegelewski, Michael
AU - Neethling, Francisca A.
N1 - Funding Information:
This work was supported in part by a fellowship from the American Committee for the Weizmann Institute of Science and by various private donors and the Adobe Animal Hospital, Los Altos, CA 94022.
PY - 2013
Y1 - 2013
N2 - This unit describes the production of monoclonal antibodies beginning with immunization, cell fusion, and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of theseBcell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium. An alternate protocol describes cell fusion and one-step selection and cloning of hybridomas utilizing a semi-solid methylcellulosebased medium (ClonaCell-HY from StemCell Technologies).
AB - This unit describes the production of monoclonal antibodies beginning with immunization, cell fusion, and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of theseBcell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium. An alternate protocol describes cell fusion and one-step selection and cloning of hybridomas utilizing a semi-solid methylcellulosebased medium (ClonaCell-HY from StemCell Technologies).
KW - B cell hybridomas
KW - Limiting dilution
KW - Semi-solid methylcellulose-based medium
KW - monoclonal antibodies
UR - http://www.scopus.com/inward/record.url?scp=84896139052&partnerID=8YFLogxK
U2 - 10.1002/0471142735.im0205s102
DO - 10.1002/0471142735.im0205s102
M3 - Article
C2 - 24510488
AN - SCOPUS:84896139052
SN - 1934-3671
JO - Current Protocols in Immunology
JF - Current Protocols in Immunology
IS - SUPPL.102
M1 - 2.5
ER -