TY - JOUR
T1 - Production of monoclonal antibodies.
AU - Yokoyama, Wayne M.
AU - Christensen, Michelle
AU - Santos, Gary Dos
AU - Miller, Diane
N1 - Funding Information:
This work was supported in part by a fellowship from the American Committee for the Weizmann Institute of Science and by various private donors and the Adobe Animal Hospital, Los Altos, CA 94022.
PY - 2006/9
Y1 - 2006/9
N2 - This unit describes the production of monoclonal antibodies beginning with immunization and cell fusion and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium. An alternate protocol describes cell fusion and one-step selection and cloning of hybridomas utilizing a semi-solid methylcellulose-based medium (ClonaCell-HY).
AB - This unit describes the production of monoclonal antibodies beginning with immunization and cell fusion and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium. An alternate protocol describes cell fusion and one-step selection and cloning of hybridomas utilizing a semi-solid methylcellulose-based medium (ClonaCell-HY).
UR - http://www.scopus.com/inward/record.url?scp=46449086546&partnerID=8YFLogxK
U2 - 10.1002/0471142735.im0205s74
DO - 10.1002/0471142735.im0205s74
M3 - Article
C2 - 18432969
AN - SCOPUS:46449086546
SN - 1934-3671
VL - Chapter 2
SP - Unit 2.5
JO - Current protocols in immunology / edited by John E. Coligan ... [et al.]
JF - Current protocols in immunology / edited by John E. Coligan ... [et al.]
ER -