Production of immunogenic West Nile virus-like particles using a herpes simplex virus 1 recombinant vector

Travis J. Taylor, Fernando Diaz, Robert C. Colgrove, Kristen A. Bernard, Neal A. DeLuca, Sean P.J. Whelan, David M. Knipe

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

West Nile virus (WNV) is a flavivirus that swept rapidly across North America in 1999, declined in prevalence, and then resurged in 2012. To date, no vaccine is available to prevent infection in the human population. Herpes simplex virus (HSV) replication-defective vaccine vectors induce a durable immunity characterized by strong antibody and CD8+ T cell responses even in HSV-immune animals. In this study, a WNV protein expression cassette was optimized for virus-like particle (VLP) production in transfection studies, and the cassette was recombined into an HSV-1 d106-WNV virus vector, which produced extracellular VLPs, as confirmed by immunoelectron microscopy. Immunization of mice with the d106-WNV recombinant vector elicited a specific anti-WNV IgG response. This study highlights the flavivirus coding sequences needed for efficient assembly of virus-like particles. This information will facilitate generation of additional vaccine vectors against other flaviviruses including the recently emerged Zika virus.

Original languageEnglish
Pages (from-to)186-193
Number of pages8
JournalVirology
Volume496
DOIs
StatePublished - Sep 1 2016

Keywords

  • Flavivirus
  • HSV recombinant vector
  • Vaccine vector
  • West Nile virus
  • Zika virus

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