Production of glucaric acid from a synthetic pathway in recombinant Escherichia coli

Tae Seok Moon, Sang Hwal Yoon, Amanda M. Lanza, Joseph D. Roy-Mayhew, Kristala L. Jones Prather

Research output: Contribution to journalArticlepeer-review

208 Scopus citations

Abstract

A synthetic pathway has been constructed for the production of glucuronic and glucaric acids from glucose in Escherichia coli. Coexpression of the genes encoding myo-inositol-1-phosphate synthase (Ino1) from Sac-charomyces cerevisiae and myo-inositol oxygenase (MIOX) from mice led to production of glucuronic acid through the intermediate myo-inositol. Glucuronic acid concentrations up to 0.3 g/liter were measured in the culture broth. The activity of MIOX was rate limiting, resulting in the accumulation of both myo-inositol and glucuronic acid as final products, in approximately equal concentrations. Inclusion of a third enzyme, uronate dehydrogenase (Udh) from Pseudomonas syringae, facilitated the conversion of glucuronic acid to glucaric acid. The activity of this recombinant enzyme was more than 2 orders of magnitude higher than that of Ino1 and MIOX and increased overall flux through the pathway such that glucaric acid concentrations in excess of 1 g/liter were observed. This represents a novel microbial system for the biological production of glucaric acid, a "top value-added chemical" from biomass.

Original languageEnglish
Pages (from-to)589-595
Number of pages7
JournalApplied and Environmental Microbiology
Volume75
Issue number3
DOIs
StatePublished - Feb 2009

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