Production of an EGFR targeting molecule from a conditionally replicating adenovirus impairs its oncolytic potential

Akseli Hemminki, Minghui Wang, Tanja Hakkarainen, Renee A. Desmond, Jarmo Wahlfors, David T. Curiel

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Oncolytic virotherapy with conditionally replicating viruses is a promising approach for treating advanced cancers. Promiscuous tropism and low tumor transduction have represented limiting issues, which targeting approaches seek to overcome. An approach utilizing a secretory targeting molecule for the epidermal growth factor pathway (sCAR-EGF) has previously been shown to be compatible with replicating adenoviruses, when an E1-deleted vector was used in a dual-virus system in conjunction with a replication-competent agent. Here, we constructed a virus that replicates in cancer cells and codes for sCAR-EGF. Interestingly, the oncolytic potency of the novel agent was not improved over nontargeted controls in vitro or in vivo. These results suggest that the expression of biologically active proteins can be counterproductive to virus replication.

Original languageEnglish
Pages (from-to)583-588
Number of pages6
JournalCancer gene therapy
Volume10
Issue number8
DOIs
StatePublished - Aug 1 2003
Externally publishedYes

Keywords

  • Adenovirus
  • Biological therapy
  • Epidermal growth factor

Fingerprint Dive into the research topics of 'Production of an EGFR targeting molecule from a conditionally replicating adenovirus impairs its oncolytic potential'. Together they form a unique fingerprint.

  • Cite this