Production, characterization and use of five monoclonal antibodies to human IL-4

M. G. Scott; A. D. Levine; A. W. Butch; M. B. Bowen; K. A. Macke; M. H. Nahm

Production, characterization and use of five monoclonal antibodies to human IL-4

M. G. Scott, A. D. Levine, A. W. Butch, M. B. Bowen, K. A. Macke, M. H. Nahm

Division of Laboratory and Genomic Medicine

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We describe five murine monoclonal antibodies that are specific for human IL-4. At least three spatially distinct epitopes on the hIL-4 molecule are recognized by this panel of antibodies which allowed development of a sensitive sandwich EIA specific for hIL-4. The EIA is capable of detecting 200 pg/ml of hIL-4 and exhibits no detectable crossreactivity to seven other human cytokines examined. In addition to the EIA these antibodies are also useful in a number of other techniques for investigating hIL-4. Recombinant hIL-4 can be easily and efficiently purified by affinity chromatography using these mAbs. Western blotting with two of the antibodies detects as little as 1.8 ng/ml of hIL-4 in a 50 μl sample. Finally, we present a method for cytoplasmic localization of hIL-4 by a fluorescence staining method utilizing a hIL-4 specific mAb.

Original language	English
Pages (from-to)	81-93
Number of pages	13
Journal	Lymphokine Research
Volume	9
Issue number	1
State	Published - Jan 1 1990

Cite this

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title = "Production, characterization and use of five monoclonal antibodies to human IL-4",

abstract = "We describe five murine monoclonal antibodies that are specific for human IL-4. At least three spatially distinct epitopes on the hIL-4 molecule are recognized by this panel of antibodies which allowed development of a sensitive sandwich EIA specific for hIL-4. The EIA is capable of detecting 200 pg/ml of hIL-4 and exhibits no detectable crossreactivity to seven other human cytokines examined. In addition to the EIA these antibodies are also useful in a number of other techniques for investigating hIL-4. Recombinant hIL-4 can be easily and efficiently purified by affinity chromatography using these mAbs. Western blotting with two of the antibodies detects as little as 1.8 ng/ml of hIL-4 in a 50 μl sample. Finally, we present a method for cytoplasmic localization of hIL-4 by a fluorescence staining method utilizing a hIL-4 specific mAb.",

author = "Scott, {M. G.} and Levine, {A. D.} and Butch, {A. W.} and Bowen, {M. B.} and Macke, {K. A.} and Nahm, {M. H.}",

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T1 - Production, characterization and use of five monoclonal antibodies to human IL-4

AU - Scott, M. G.

AU - Levine, A. D.

AU - Butch, A. W.

AU - Bowen, M. B.

AU - Macke, K. A.

AU - Nahm, M. H.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - We describe five murine monoclonal antibodies that are specific for human IL-4. At least three spatially distinct epitopes on the hIL-4 molecule are recognized by this panel of antibodies which allowed development of a sensitive sandwich EIA specific for hIL-4. The EIA is capable of detecting 200 pg/ml of hIL-4 and exhibits no detectable crossreactivity to seven other human cytokines examined. In addition to the EIA these antibodies are also useful in a number of other techniques for investigating hIL-4. Recombinant hIL-4 can be easily and efficiently purified by affinity chromatography using these mAbs. Western blotting with two of the antibodies detects as little as 1.8 ng/ml of hIL-4 in a 50 μl sample. Finally, we present a method for cytoplasmic localization of hIL-4 by a fluorescence staining method utilizing a hIL-4 specific mAb.

AB - We describe five murine monoclonal antibodies that are specific for human IL-4. At least three spatially distinct epitopes on the hIL-4 molecule are recognized by this panel of antibodies which allowed development of a sensitive sandwich EIA specific for hIL-4. The EIA is capable of detecting 200 pg/ml of hIL-4 and exhibits no detectable crossreactivity to seven other human cytokines examined. In addition to the EIA these antibodies are also useful in a number of other techniques for investigating hIL-4. Recombinant hIL-4 can be easily and efficiently purified by affinity chromatography using these mAbs. Western blotting with two of the antibodies detects as little as 1.8 ng/ml of hIL-4 in a 50 μl sample. Finally, we present a method for cytoplasmic localization of hIL-4 by a fluorescence staining method utilizing a hIL-4 specific mAb.

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M3 - Article

C2 - 1691418

AN - SCOPUS:0025219341

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JO - Lymphokine Research

JF - Lymphokine Research

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ER -

Production, characterization and use of five monoclonal antibodies to human IL-4

Abstract

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