The use of H/D exchange, MS/MS and ESI-MS for determining the peptide/peptide interactions and structure and metal ion binding of gramicidin dimer were discussed. The H/D exchange was done by diluting a concentrated solution of gramicidin in the protonated solvent with the deuterated solvent and immediately injecting the solution into the mass spectrometer. It was observed that the stability of the metal-ion-bound dimer decreases with increasing ionic radius of the metal ion. It was suggested that the metal ion binds on the inside of the dimer in such a way that larger metal ions push apart the monomers, weakening the dimer.
|Number of pages
|Published - 2002
|Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002 → Jun 6 2002
|Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
|06/2/02 → 06/6/02